| 高效离子交换色谱法测定埃本膦酸钠 |
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| 引用本文： | 胡名扬,周杏琴,王博诚.高效离子交换色谱法测定埃本膦酸钠[J].色谱,2000,18(3):254-255. |
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| 作者姓名： | 胡名扬 周杏琴 王博诚 |
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| 作者单位： | 江苏省原子医学研究所核医学国家重点实验室!江苏无锡214063 |
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| 基金项目： | 国家“九五”攻关项目 !(批准号 96- 90 6- 0 5- 0 5) |
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| 摘 要： | 采用高效离子交换色谱法,在流动相中加入二价铜离子,利用埃本膦酸钠通过流动相时与铜离子能形成紫外吸收的络合物的性质,在240nm波长下检测,所用柱为DEAE离子交换柱。方法的最低检测质量浓度为6mg/L线性回归系数为0.9989。方法简便,重现性好。同时考察了pH及有机溶剂对平衡分配的影响。
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| 关 键 词： | 高效离子交换色谱法 埃本膦酸钠 络合物 |
Determination of Ibandronate by High Performance Ion Exchange Chromatography |
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| HU Ming-yang,ZHOU Xing-qin,WANG Bo-cheng.Determination of Ibandronate by High Performance Ion Exchange Chromatography[J].Chinese Journal of Chromatography,2000,18(3):254-255. |
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| Authors: | HU Ming-yang ZHOU Xing-qin WANG Bo-cheng |
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| Institution: | National Laboratory of Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China. |
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| Abstract: | A method for the determination of Ibandronate by high performance ion exchange chromatography is reported.Ibandronate can form a complex compound with the copper ion added in the mobile phase.The complex can be detected at UV 240 nm.The effects of pH of mobile phase and concentration of methanol to the retention time wrer studied.Concentration of 10% methanol and pH 2.5 wre suitable for the detection in less than 5min.The flow rate was 1.0 mL/min.The relative standard deviation of peak area was 0.8%.The linear correlation was observed from 300 mg/L to 1 500mg/L of Ibandronate (r=0.998 9).The lowest detectable concentration of Ibandronate was 6 mg/l.The method is simple,rapid and reproducible. |
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| Keywords: | high performance ion exchange chromatography Ibandronate complex |
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