| 血红蛋白在纳米孔径氧化铝膜修饰电极上的直接电化学 |
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| 引用本文： | 武五爱,郭满栋,尹志芬,尉景瑞,李婧芳,李君,张宇.血红蛋白在纳米孔径氧化铝膜修饰电极上的直接电化学[J].化学学报,2009,67(8):781-785. |
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| 作者姓名： | 武五爱 郭满栋 尹志芬 尉景瑞 李婧芳 李君 张宇 |
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| 作者单位： | 山西师范大学化学系,临汾,041004 |
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| 摘 要： | 运用电化学包埋法成功将血红蛋白(Hb)固定于纳米孔阳极氧化铝膜(AAO)修饰的玻碳电极(GC)表面, 制得Hb/PPy/AAO/GC修饰电极, 并对Hb/PPy/AAO/GC修饰电极的制备条件进行了优化. 研究了Hb/PPy/AAO/GC修饰电极在磷酸缓冲液(pH＝6.8)中的电化学行为, 探讨了血红蛋白在AAO修饰电极表面的直接电子转移机理. 结果表明阳极氧化铝膜不仅保持了血红蛋白的生物活性, 而且通过它的纳米尺寸效应, 实现了Hb与电极之间的直接电子转移. 其研究内容对生命科学和临床医学有着重要意义.
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| 关 键 词： | 血红蛋白 氧化铝膜 化学修饰电极 |
| 收稿时间： | 2008-8-23 |
| 修稿时间： | 2008-10-28 |
Direct Electrochemistry of Hemoglobin Immobilized on Glassy Carbon Electrode Modified with Anodic Alumina Oxide Films |
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| Wu,Wuai,Guo,Mandong,Yin,Zhifen,Wei,Jingrui,Li,Jingfang,Li,Jun,Zhang,Yu.Direct Electrochemistry of Hemoglobin Immobilized on Glassy Carbon Electrode Modified with Anodic Alumina Oxide Films[J].Acta Chimica Sinica,2009,67(8):781-785. |
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| Authors: | Wu Wuai Guo Mandong Yin Zhifen Wei Jingrui Li Jingfang Li Jun Zhang Yu |
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| Institution: | Department of Chemistry;Shanxi Normal University;Linfen 041004 |
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| Abstract: | A steady Hb/PPy/AAO/GC electrode was obtained by successfully immobilizing hemoglobin(Hb) on a glassy carbon(GC) electrode,which was modified using anodic alumina oxide(AAO) films.Its optimum experimental conditions were found.The electrochemistry behavior of the Hb/PPy/AAO/GC biosensor in PBS(pH=6.8) and the direct electron transfer mechanism of Hb were discussed,finding that the anodic alumina oxide films kept the biological activity of Hb.Moreover through nano-size effect of the films,the direct electron... |
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| Keywords: | hemoglobin anodic alumina oxide films chemical modified electrode |
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