碳纤维汞膜微电极1.5次微分伏安法测定米托蒽醌的研究

米托蒽醌（ＭＸＴ）能吸附在碳纤维汞膜微电极表面，阴极溶出时，产生一个灵敏的１．５次微分溶出峰，其峰电流和米托葱酿的浓度在１．０×１０－５ｍｏｌ／Ｌ～１．３×１０－９ｍｏｌ／Ｌ范围内具有良好的线性关系，经３ｍｉｎ的富集，检出限可达１．０×１０－１０ｍｏｌ／Ｌ，据此建立了测定ＭＸＴ的灵敏的新方法，本文讨论了实验条件，并对血样中的ＭＸＴ进行了测定。     

血红蛋白在纳米孔径氧化铝膜修饰电极上的直接电化学

运用电化学包埋法成功将血红蛋白(Hb)固定于纳米孔阳极氧化铝膜(AAO)修饰的玻碳电极(GC)表面, 制得Hb/PPy/AAO/GC修饰电极, 并对Hb/PPy/AAO/GC修饰电极的制备条件进行了优化. 研究了Hb/PPy/AAO/GC修饰电极在磷酸缓冲液(pH＝6.8)中的电化学行为, 探讨了血红蛋白在AAO修饰电极表面的直接电子转移机理. 结果表明阳极氧化铝膜不仅保持了血红蛋白的生物活性, 而且通过它的纳米尺寸效应, 实现了Hb与电极之间的直接电子转移. 其研究内容对生命科学和临床医学有着重要意义.     

铟(Ⅲ)-向红菲啰啉络合物的吸附伏安法研究

运用1 5次微分伏安法 ,常规溶出伏安法及循环伏安法研究了铟(Ⅲ) -向红菲啉络合物在汞电极上的电化学行为 ,讨论了络合物的形成机理及条件 ,建立了测试痕量铟的新方法 ,运用该法对样品中痕量铟进行了测试 ,结果令人满意。     

碳纤维汞膜微电极1.5次微分伏安法测定胆固醇的研究

将胆固醇吸附在碳纤维汞膜微电极表面，阳极溶出时，可产生一个灵敏的１．５次微分溶出峰，其峰电位为－０．０６Ｖ（ｖｓ，ＳＣＥ，下同），峰电流和样品浓度在１．０×１０－５～１．０×１０－７ｍｏｌ／Ｌ范围内有良好的线性关系。检出限可达１．０×１０－８ｍｏｌ／Ｌ。本文讨论了测定条件，建立了测定胆固醇的新方法，并对实际样品进行了分析。     

漆酶电极的研制及应用

本文报道二茂铁修饰电极为基底的漆酶电极的研制及应用，讨论了电极性能及响应机理，找出了实验最佳条件。并对环境污水中对苯二酚含量进行规定。一结果令人满意。     

Electrochemical detection of short sequences related to the hepatitis B virus using MB on chitosan-modified CPE

A novel electrochemical DNA biosensor based on methylene blue (MB) and chitosan-modified carbon paste electrode (CCPE) for short DNA sequences and polymerase chain reaction (PCR) amplified real samples related to the hepatitis B virus (HBV) hybridization detection is presented. Differential pulse voltammetry (DPV) was used to investigate the surface coverage and hybridization event. The decrease in the peak current of MB, an electroactive label, was observed upon hybridization of probe with the target. Numerous factors affecting the target hybridization and indicator binding reaction are optimized to maximize the sensitivity.     

Preparation of Molecularly Imprinted Electrochemical Sensor for Detection of Vincristine Based on Reduced Graphene Oxide/Gold Nanoparticle Composite Film

An innovative molecularly imprinted electrochemical sensor was fabricated based on reduced graphene oxide (RGO) and gold nanocomposite (Au) for rapid detection of vincristine (VCR). The RGO‐Au composite membrane was obtained via direct one‐step electrodeposition technique of graphene oxide (GO) and chloroauric acid (HAuCl₄) on the surface of a glassy carbon electrode (GCE) by means of cyclic voltammetry (CV) in the potential range between ?1.5 and 0.6 V in phosphate buffer solution (PBS) of pH 9.18, which is capable of effectively utilizing its superior electrical conductivity, larger specific surface area due to its synergistic effect between RGO and Au. The molecularly imprinted polymers (MIPs) were synthesized on the RGO‐Au modified glassy carbon electrode surface with VCR as the template molecular, methyl acrylic acid (MAA) as the functional monomer, and ethylene glycol maleic rosinate acrylate (EGMRA) as a cross‐linker. The performance of the sensor was investigated by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) in detail. Under the optimum conditions, the fabricated sensor exhibited a linear relationship between oxidation peak current and VCR concentration over the range of 5.0×10^?8–5.0×10^?6 mol·L ^minus;1 with a correlation coefficient of 0.9952 and a detection limit (S/N=3) of 2.6×10^minus;8 mol·L^minus;1. The results indicated that the imprinted polymer films exhibited an excellent selectivity for VCR. The imprinted sensor was successfully used to determine VCR in real samples with recoveries of 90% –120% by using the standard addition method.     

肌酐-铜(Ⅱ)体系的电化学氧化及分析应用

肌酐能够和Cu~(2+)生成络合物,该络合物可以吸附于玻碳电极表面,在pH3.50的NH_4Cl-HCl底液中,吸附于电极表面的肌酐-Cu~(2+)络合物在较负的电位下还原为肌酐-Cu~+,当电极向正向扫描时,产生一个灵敏的表面催化波,其峰电流和肌酐的浓度在1.0×10~(-5)～1.0×10~(-8)mol/L范围内有良好的线性关系,其检出限为1.0×10~(-9)mol/L(富集5min).研究了肌酐-Cu~(2+)络合物在玻碳电极上的氧化还原机理,选择了测定肌酐的最佳实验条件,建立了测定肌酐的新的灵敏的方法.     

痕量钴的分析

综述了近年来测定痕量钴的进展状况,展望了测定痕量钴的要求及发展方向,包括光度法,色谱法及质谱法。