高效液相色谱手性流动相添加剂分离乳酸对映体

分别将β-环糊精、2，6－二甲基－β－环瑚精和2，3，6－三甲基－β－环糊精作为手性流动相添加剂，系统地研究了D，L-乳酸在反相HPLC系统中的拆分，考察了流动相种类，pH值和手性流动相添加剂的浓度对手性分离的影响，建立了甲基化β-环糊精动态手性固定相分离乳酸对映体的方法。

High Performance Liquid Chromatographic Separation ofLactic acid Enantiomer Using Chiral Mobile Phase Additives

The enantiomeric resolution of lactic acid was studied by reversed high performance liquid chromatography. β-cyclodextrin (β-CD), dimethyl (DM) and TM-β-CD were used as chiral mobile phase additives. The effects of different mobile phase, pH and concentration of chiral mobile phase additives on resolution of D,L-lactic acid were investigated. The results showed: the enantioselectivity of TM-β-CD was better than those of β-CD and DM-β-CD. D,L-lactic acid could be partly separated by DM-β-CD 　and could not be separated by β-CD, while it could be fully separated by TM-β-CD. As the concentration of TM-β-CD changed from 0.20　mmol/L to 1.00　mmol/L, The resolution varied slightly. The experiment of the effect of pH of mobile phase on Rs showed: as pH changed from 2.4 to 3.0, the Rs remain stable, and when pH＞3.1, the Rs decreased with the increase of pH. The RSD of retention time of L-lactic acid and D-lactic acid were 0.2% and 0.2%, respectively. The chromatographic systems with a dynamically-generated stationary phase with 2,3,6-tri-O-methyl-β-cyclodextrin proved to be an effective method for lactic acid enantiomer separation