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| 快速高分离液相色谱-串联质谱法检测原乳中活性β-内酰胺酶 | |
| 引用本文： | 孙汉文,李挥,张敬轩,周正.快速高分离液相色谱-串联质谱法检测原乳中活性β-内酰胺酶[J].分析化学,2010,38(8). |
| 作者姓名： | 孙汉文 李挥 张敬轩 周正 |
| 作者单位： | 1. 河北省分析科学重点实验室,河北大学化学与环境科学学院,保定,071002 2. 河北省分析科学重点实验室,河北大学化学与环境科学学院,保定,071002;河北省食品质量监督检验研究院,石家庄,050051 3. 河北省食品质量监督检验研究院,石家庄,050051 |
| 基金项目： | 河北省科技支撑项目基金 |
| 摘 要： | 建立了原乳中活性β-内酰胺酶拮抗剂的快速高分离液相色谱-串联质谱(RRLC-MS/MS)检测方法.利用活性β-内酰胺酶能够酶解青霉素族药物的特性,在原乳中加入适量氨苄青霉素,酶解1 h后,检测原乳中氨苄青霉素酶解率,定性检测活性目标物.试样中氨苄青霉素经乙腈溶液提取,HLB固相萃取柱净化和浓缩后,RRLC-MS/MS多反应监测模式定性与定量分析.本方法对活性β-内酰胺酶检出限为4 U/mL;对原乳中添加1 mg/kg水平氨苄青霉素的加标回收率为92.3%～95.5%;相对标准偏差RSD≤10%(n=6).本方法的定性结果与生物杯碟法完全一致. |
| 关 键 词： | 液相色谱-串联质谱 原乳 β-内酰胺酶 |
Qualitative Analysis of Active Beta-Lactamases in Milk Samples by Rapid Resolution Liquid Chromatography-Tandem Mass Spectrometry |
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| SUN Han-Wen,LI Hui,ZHANG Jing-Xuan,ZHOU Zheng.Qualitative Analysis of Active Beta-Lactamases in Milk Samples by Rapid Resolution Liquid Chromatography-Tandem Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2010,38(8). | |
| Authors: | SUN Han-Wen LI Hui ZHANG Jing-Xuan ZHOU Zheng |
| Institution: | SUN Han-Wen1,LI Hui1,2,ZHANG Jing-Xuan2,ZHOU Zheng2 1(Key Laboratory of Analytical Science of Hebei,College of Chemistry and Environmental Science,Hebei University,Baoding 071002) 2(Hebei Institute of Food Quality Supervision Inspection and Research,Shijiazhuang 050051) |
| Abstract: | A rapid resolution liquid chromatography-tandem mass spectrometric method (RRLC-MS/MS) has been developed for the qualitative analysis of active beta-lactamases in milk samples. Penicillin was added to the samples solution to enzymolyze the active beta-lactamases. Then,the analytes were extracted by CH3CN-H2O from homogenized samples,and the extract was cleaned by Oasis HLB solid phase extraction cartridge. Quantification of penicillin was achieved by RRLC-MS/MS with multiple reaction monitoring. Detection ... |
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