| 新型毛细管电泳化学发光系统应用于氨基酸分离测定 |
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| 引用本文： | 尹东光,吴明红,谢春娟,张礼,刘斌虎,周小舟,王鹏.新型毛细管电泳化学发光系统应用于氨基酸分离测定[J].分析化学,2009,37(1). |
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| 作者姓名： | 尹东光 吴明红 谢春娟 张礼 刘斌虎 周小舟 王鹏 |
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| 作者单位： | 上海大学环境与化学工程学院,上海,200444 |
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| 基金项目： | 上海市浦江人才计划(D类)，上海市纳米专项项目，上海市重点学科建设项目，上海市启明星计划项目 |
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| 摘 要： | 本研究建立一种以过氧草酸酯为化学发光体系的新型毛细管电泳化学发光系统,并将该系统应用于氨基酸的分离测定.该系统设计了新型的电极模式与反应混和模式以获得高的信号稳定性与检测灵敏度.以一端与高压电源负极相连的铂丝连接到不锈钢注射针头为接地电极,电泳毛细管穿过注射针头,且其末端到达注射针头的出口,电泳毛细宇航局末端与化学发光试剂在反应池中以相对的方向靠近.实验结果表明,该系统稳定性好、分离效率高、灵敏度高、精密度好,对天冬氨酸与亮氨酸的检出限分别为2.0和 1.1 nmol/L.峰高及迁移时间的相对标准偏差分别为2.3%～3.8%和1.2%～1.5%.
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| 关 键 词： | 毛细管电泳 草酸酯 化学发光 氨基酸 |
A Novel Capillary Electrophoresis-Chemiluminescence System for Separation and Detection of Amino Acids |
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| YIN Dong-Guang,WU Ming-Hong,XIE Chun-Juan,ZHANG Li,LIU Bing-Hu,ZHOU Xiao-Zhou,WANG Peng.A Novel Capillary Electrophoresis-Chemiluminescence System for Separation and Detection of Amino Acids[J].Chinese Journal of Analytical Chemistry,2009,37(1). |
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| Authors: | YIN Dong-Guang WU Ming-Hong XIE Chun-Juan ZHANG Li LIU Bing-Hu ZHOU Xiao-Zhou WANG Peng |
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| Institution: | College of Environmental and Chemical Engineering;Shanghai University;Shanghai 200444 |
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| Abstract: | A peroxyoxalate-based chemiluminescence(CL) detection following capillary electrophoresis(CE) separations for assaying amino acids is described.To achieve high signal stability and sensivity,a new interface was designed including a new mixing mode and a new grounding electrode mode.A platinum wire-connected cathode of a high voltage source was put onto a stainless steel needle tube,which was used as grounding electrode.The CE capillary was inserted into the stainless steel needle tube and the capillary outl... |
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| Keywords: | Capillary electrophoresis peroxyoxalate chemiluminescence amino acid |
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