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| 全二维微柱液相色谱-质谱鉴定人胃癌组织中的差异蛋白质 | |
| 引用本文： | 罗福文,陶定银,赵鹏,张丽华,贾玉杰,张玉奎.全二维微柱液相色谱-质谱鉴定人胃癌组织中的差异蛋白质[J].分析化学,2009,37(10). |
| 作者姓名： | 罗福文 陶定银 赵鹏 张丽华 贾玉杰 张玉奎 |
| 作者单位： | 1. 大连医科大学病理生理教研室,大连,116027;大连医科大学附属二普通外科,大连,116027 2. 中国科学院大连化学物理研究所,大连,116023 3. 大连医科大学附属二普通外科,大连,116027 |
| 基金项目： | 国家自然科学基金重点基金，国家自然科学基金面上基金，"973"计划 |
| 摘 要： | 构建了以阳离子交换色谱-反相色谱(SCX-RPLC)为分离模式的新型全二维微柱液相色谱-质谱分离平台.采用了醋酸铵缓冲液梯度洗脱,实现了第一维肽段的分步洗脱,洗脱的肽段经富集除盐后通过接口进入反相色谱微柱,通过线性梯度实现第二维进一步分离,最后进入质谱进行检测.采用此平台分析了人胃癌组织与正常组织提取蛋白质信息,其中正常胃组织鉴定蛋白质数为537个,而癌症组织鉴定蛋白质数目为506个.对胃癌和正常组织两种提取蛋白质酶解产物的蛋白质检索结果进行比较分析,将鉴定的蛋白质按照物理性质进行分布,找出正常组织与癌症组织间蛋白质差异,筛选出一种可能发生变异的癌症特有蛋白. |
| 关 键 词： | 胃癌组织 蛋白质组 二维液相色谱-质谱联用 |
Identification of Difference of Proteins Between Human Stomach Tumor and Healthy Tissues by Two-dimensional Liquid Chromatography-Mass Spectrometry |
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| LUO Fu-Wen,TAO Ding-Yin,Zhao Peng,Zhang Li-Hua,Jia Yu-Jie,Zhang Yu-Kui.Identification of Difference of Proteins Between Human Stomach Tumor and Healthy Tissues by Two-dimensional Liquid Chromatography-Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2009,37(10). | |
| Authors: | LUO Fu-Wen TAO Ding-Yin Zhao Peng Zhang Li-Hua Jia Yu-Jie Zhang Yu-Kui |
| Institution: | LUO Fu-Wen1,2,TAO Ding-Yin3,Zhao Peng2,Zhang Li-Hua3,Jia Yu-Jie2,Zhang Yu-Kui31(Dalian Medical University,Staff Room of Pathology and Physiology,Dalian 116027)2(Dalian Medical University Second Affiliated Department of General Surgery,Dalian 116027)3(Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023) |
| Abstract: | A two-dimensional high performance liquid chromatographic separation platform based on strong cation exchange(SCX) chromatography and reversed phase(RP) chromatography was developed.Peptides were first separated by a SCX column under stepwise gradient of 0,20,200,300,500,1000 and 2000 mmol/L NH4AC buffer.After each fraction was collected and desalted by a RP trap column,further separation was implemented on a RPLC column under linear gradient elution.All the components were identified by mass spectrometry a... |
| Keywords: | Stomach cancer tissue proteomics two-dimensional high performance liquid chromatography-mass spectrometry |
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