| 电泳芯片的制作及其进样与分离 |
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| 引用本文： | 汤扬华,叶雄英,周兆英,冯焱颍,金亚.电泳芯片的制作及其进样与分离[J].分析化学,2001,29(5):606-610. |
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| 作者姓名： | 汤扬华 叶雄英 周兆英 冯焱颍 金亚 |
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| 作者单位： | 1. 清华大学精密仪器系,
2. 清华大学精密化学系, |
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| 基金项目： | 国家自然科学基金 (No .2 983 3 0 70 )，国家重点基础研究发展规划资助项目 (No .G19990 3 3 10 6) |
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| 摘 要： | 利用微细加工技术研究在玻璃上制作电泳芯片的方法，测试了微管道的伏安特性曲线。在该电泳芯片上进行了注样和分离实验，采用激光诱导荧光法进行检测，利用CCD拍摄了进样和分离的全过程。分析了电泳芯片上施加不同的电压对样品注样的影响，给出了FITC－OH和FITC－Arg分离谱图。
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| 关 键 词： | 湿法刻蚀 电泳芯片 激光诱导荧光 微细加工技术 电化学检测 进样 分离 |
Glass Chips for Capillary Electrophoresis: Micromachining,Sample Injection and Separation |
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| Tang Yanghua,YE Xiongying,Zhou Zhaoying,Feng Yanying,Jin Ya.Glass Chips for Capillary Electrophoresis: Micromachining,Sample Injection and Separation[J].Chinese Journal of Analytical Chemistry,2001,29(5):606-610. |
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| Authors: | Tang Yanghua YE Xiongying Zhou Zhaoying Feng Yanying Jin Ya |
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| Abstract: | Micromachining of capillary electrophoresis chips is described. The current in channels was determined versus the voltage applied on the chip. Using laser induced fluorescence detection, the process of injection and separation on chips was recorded with charge coupled device (CCD). With defferent voltge of reservoirs on glass chips, profiles of the sample plug were studied. The elctropherogram of hydrolysis product of fluorescein isothiocyanate (FITC OH) and arginine labeled by fluorescein isothiocyante(FITC Arg) was obtained. |
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| Keywords: | Wet etching bonding capillary electrophoresis chip laser induced fluorescence |
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