大孔吸附树脂分离纯化异甘草素的研究

研究大孔吸附树脂分离纯化异甘草素的工艺条件及参数。通过研究HPD-600、D4020、D101、AB-8、NKA-II、AL-2和NKA-9树脂对异甘草素的吸附和解吸附能力,筛选最佳树脂为AB-8,并研究了其对异甘草素的吸附和解吸附性能,确定了最佳的吸附与解吸附工艺参数,吸附:pH=5,室温,流速1.5BV/h,溶液处理量为5BV;脱附:洗脱剂为70%的乙醇溶液,流速1BV/h,洗脱剂用量4.5BV。异甘草素样品溶液经AB-8树脂吸附与脱附后回收率为76.7%,纯度由2.02%提高到29.1%,提高了14.4倍。实验结果表明,AB-8树脂对异甘草素的吸附量大,脱附容易,可以应用于异甘草素的分离纯化。

THE STUDY ON SEPARATION AND PURIFICATION OF ISOLIQUIRITIGENIN BY MACROPOROUS ADSORPTION RESIN

The process parameters of the separation and purification of isoliquiritigenin with macroporous adsorption resins were studied.Seven different kinds of macroporous adsorption resins named HPD-600,D4020,D101,AB-8,NKA-II,AL-2 and NKA-9 were studied on the static capacity of adsorption and desorption,the better resin was AB-8.The optimum adsorption and desorption parameters of separation and purification isoliquiritigenin with resin AB-8 as follows,adsorption: room temperature,flow rate 1.5BV/h,volume 5BV;desorption: elution solvent 70% alcohol,flow rate 1BV/h,elution volume 4.5BV.After treated with AB-8,the yield of isoliquiritigenin was 76.7%,and the purity was increased from 2.02% to 29.1% with 14.4 times as high as the crude extracts.The results showed that resin AB-8 had good ability of separation and purification of isoliquiritigenin,it could be used to separate and purify isoliquiritigenin.