游离和膜固定化脂肪酶在双液相介质中催化二肽合成的研究

以N－乙酰－L－苯丙氨酸乙酯和L－亮氨酰胺、L－丙氨酰胺、L－苯丙氨酰胺 、L－缬氨酰胺等L－氨基酰胺为底物，以游离或膜固定化脂肪酶为催化剂，在水、 有机溶剂单相和水－有机相双相体系反应介质中，合成了N－乙酰－L－苯丙氨酰基 －L－亮氨酰胺、N－乙酰－L－苯丙氨酰基－L－丙氨酰胺、N－乙酰－L－苯丙氨酰 基－L－苯丙氨酰胺、N－乙酰－L－苯丙氨酰基－L－缬氨酰胺等二肽。研究证明， 由于反应和纯化的同步进行，二肽可以在双液相酶膜反应器中达到高纯度和高收率 合成。

Study on dipeptide synthesis using free and membrane-bound lipase

Three dipeptides N-Ac-L-Phe-L-Leu-NH 2, N-Ac-L-Phe-L-Ala-NH 2, and N-Ac-L-Phe-L-Val-NH 2 were synthesized from N-Ac-L-Phe-OEt as the carboxyl terminal substrate and L-Leu-NH 2, L-Ala-NH 2 and L-Val-NH 2 as the amino terminal substrate in either aqueous or organic solvent phase as well as aqueous-organic solvent biphase system by the free and membrane immobilized lipase. It was proved that the dipeptides could be synthesized through the two-liquid-phase enzyme membrane reaction with high yield and purity due to the simultaneous reaction and purification.