毛细管电泳法手性拆分4种氨酸和2种手性药物对映体

以双(6-氧-β-羧甲基-1,4-丁烯二酸单酯)-β-环糊精(DOCB-β-CD）作为手性添加剂，利用毛细管电泳对氨基酸和手性药物对映体进行拆分研究。 以20 mmol/L磷酸盐为缓冲溶液，考察了手性添加剂的浓度及缓冲溶液的pH值与分离电压等对拆分效果的影响，并在其优化条件下，实现了4种DL-氨基酸（苯丙氨酸、色氨酸、酪氨酸和组氨酸）以及手性药物（罗格列酮和酮洛芬）对映体的基线分离。

Chiral Separation of Enantiomers of Four Amino Acids and Two Chial Drugs through Capillary Electrophoresis

Enantiomers of amino acids and chiral drugs were separated by capillary zone electrophoresis using double (6-O-β-carboxy-methyl-1,4-maleic acid ester)-β-cyclodextrin(DOCB-β-CD) as chiral selector. The buffer solution used was 20 mmol/L phosphate. The influences of DOCB-β-CD concentration, background buffer pH value, temperature and applied voltage were investigated. Under the respective optimum condition, enantiomers separation of four kinds of DL-amino acids(Phenylalanine, Tryptophan, Tyrosine and Histidine) and two kinds of chiral drugs(rofecoxib and rosiglitazone) were attained with a clear separation from the baseline.