| ESTABLISHMENT OF A GROUP OF MURINE LEUKEMIC CELL LINES AND INVESTIGATION OF THEIR BIOLOGICAL CHARACTERISTICS |
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| 作者姓名： | 万景华 张淑靖 糜静娴 齐淑玲 吴克复 甘芾 杨保青 徐丽娟 许良中 |
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| 作者单位： | Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin，Institute of Hematology，Chinese Academy of Medical Sciences，Tianjin，Tumor Hospital，Shanghai Medical University |
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| 基金项目： | the National Natural Science Foundation of China. |
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| 摘 要： | An in vitro-vivo technique for establishment of cell lines on murine leukemia has beendeveloped. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, B lymphocytic leukemia P 388-86 and Friend erythroleu-kemia FLCL cell lines have been established. Incidence of leukemia with these cell lines was 100%. Along with the increase of genera-tions of cell lines, cell growth accelerated, generation time shortened and cloning efficienciesrose. A following up electron microscopic observation on L7811-85 and L7212-85 showed thatthe virus particles were "A" particles in original cells. When they became cell lines in vitro,virus particles increased and transformed into typical "C" particles with budding. An inhibitory activity relevant to leukemic cells on proliferation of leukemic cells hasbeen observed in the supernatant of L7811-85 medium and was regarded as an "autocrine".
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ESTABLISHMENT OF A GROUP OF MURINE LEUKEMIC CELL LINES AND INVESTIGATION OF THEIR BIOLOGICAL CHARACTERISTICS |
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| WAN JING-HUA,ZHANG SHU-JING,MI JING-XIAN,QI SHU-LING,WU KE-FU,GAN FEI,YANG BAO-QING,XU LI-JUAN,XU LIANG-ZHONG Institute of Hematology,Chinese Academy of Medical Sciences,Tianjin Tumor Hospital,Shanghai Medical University.ESTABLISHMENT OF A GROUP OF MURINE LEUKEMIC CELL LINES AND INVESTIGATION OF THEIR BIOLOGICAL CHARACTERISTICS[J].Science in China(Chemistry),1989(9). |
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| Authors: | WAN JING-HUA ZHANG SHU-JING MI JING-XIAN QI SHU-LING WU KE-FU GAN FEI YANG BAO-QING XU LI-JUAN XU LIANG-ZHONG Institute of Hematology Chinese Academy of Medical Sciences Tianjin Tumor Hospital Shanghai Medical University |
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| Abstract: | An in vitro-vivo technique for establishment of cell lines on murine leukemia has beendeveloped. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, B lymphocytic leukemia P 388-86 and Friend erythroleu-kemia FLCL cell lines have been established. Incidence of leukemia with these cell lines was 100%. Along with the increase of genera-tions of cell lines, cell growth accelerated, generation time shortened and cloning efficienciesrose. A following up electron microscopic observation on L7811-85 and L7212-85 showed thatthe virus particles were "A" particles in original cells. When they became cell lines in vitro,virus particles increased and transformed into typical "C" particles with budding. An inhibitory activity relevant to leukemic cells on proliferation of leukemic cells hasbeen observed in the supernatant of L7811-85 medium and was regarded as an "autocrine". |
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| Keywords: | leukemic cell lines in vitro-vivo technique virus particles autocrine |
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