ESTABLISHMENT OF A GROUP OF MURINE LEUKEMIC CELL LINES AND INVESTIGATION OF THEIR BIOLOGICAL CHARACTERISTICS

An in vitro-vivo technique for establishment of cell lines on murine leukemia has beendeveloped. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, B lymphocytic leukemia P 388-86 and Friend erythroleu-kemia FLCL cell lines have been established. Incidence of leukemia with these cell lines was 100%. Along with the increase of genera-tions of cell lines, cell growth accelerated, generation time shortened and cloning efficienciesrose. A following up electron microscopic observation on L7811-85 and L7212-85 showed thatthe virus particles were "A" particles in original cells. When they became cell lines in vitro,virus particles increased and transformed into typical "C" particles with budding. An inhibitory activity relevant to leukemic cells on proliferation of leukemic cells hasbeen observed in the supernatant of L7811-85 medium and was regarded as an "autocrine".     

一组小鼠白血病细胞系的建立及生物学特性的研究

本支建立了一种经PHA-脾条件培养液刺激,在体外形成白血病干细胞克隆后,再经体内传代,发病后在体外连续培养建成白血病细胞系的新方法。采用此方法成功地建立了T抑制淋巴细胞白血病L7811-85,L7212-85细胞系,非T非B淋巴白血病L1210-86,B淋巴白血病P388-86及红白血病FLCL细胞系等。对上述细胞系进行了较系统的生物学特性研究。电子显微镜观察中发现,白血病细胞的病毒颗粒在体外传代时A型颗粒消失,C型颗粒出现。并发现在L7811-85细胞系培养上清液分离的组分中,有白血病相关抑制活性的存在。