| Enzyme-catalyzed reaction of OPD-H_2O_2-HRP voltammetric enzyme-linked immunoassay system |
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| 作者姓名： | 焦奎 孙刚 张书圣 |
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| 作者单位： | Department of Applied Chemistry，Qingdao Institute of Chemical Technology，Qingdao 266042，China |
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| 基金项目： | theNationalNaturalScienceFoundationofChina |
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| 摘 要： | Ｈｏｒｓｅｒａｄｉｓｈｐｅｒｏｘｉｄａｓｅ(ＨＲＰ)ｃａｎｓｔｒｏｎｇｌｙｃａｔａｌｙｚｅｔｈｅｒｅａｃｔｉｏｎｏｆＨ2Ｏ2ｏｘｉｄｉｚｉｎｇｏｐｈｅｎｙｌｅｎｅｄｉａｍｉｎｅ(ＯＰＤ),ｔｈｅｃｈｒｏｍａｔｉｃｐｒｏｄｕｃｔｏｆｗｈｉｃｈｃａｎｂｅｄｅｔｅｃｔｅｄｉｎｅｎｚｙｍｅｌｉｎｋｅｄｉｍｍｕｎｏｓｏｒｂｅｎｔａｓｓａｙ(ＥＬＩＳＡ)ｗｉｔｈｓｐｅｃｔｒｏｐｈｏｔｏｍｅｔｒｙ1].Ｉｎｖｏｌｔａｍｍｅｔｒｉｃｅｎｚｙｍｅｌｉｎｋｅｄｉｍｍｕｎｏａｓｓａｙ2—4],ＯＰＤＨ2Ｏ2ＨＲＰｓｙｓｔｅｍｈａｓｆ…
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Enzyme-catalyzed reaction of OPD-H_2O_2-HRP voltammetric enzyme-linked immunoassay system |
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| JIAO Kui,SUN Gang and ZHANG Shusheng.Enzyme-catalyzed reaction of OPD-H_2O_2-HRP voltammetric enzyme-linked immunoassay system[J].Science in China(Chemistry),1998(4). |
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| Authors: | JIAO Kui SUN Gang and ZHANG Shusheng |
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| Abstract: | Enzyme catalyzed reaction of o phenylenediamine (OPD) H\-2O\-2 horseradish peroxidase (HRP) voltammetric enzyme linked immunoassay system has been studied in detail with electrochemical analysis, high performance liquid chromatography (HPLC), ultraviolet/visible (UV/Vis) spectroscopy, infrared (IR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. The pure product of H\-2O\-2 oxidizing OPD catalyzed by HRP was prepared with chemical method. The experimental results of voltammetry and HPLC indicate that only one product of enzyme catalyzed reaction has been obtained under the selected enzyme catalyzed reaction conditions. Identifications by UV/Vis spectrum, IR spectrum and 13 C NMR spectrum show that the product is 2,3 diaminophenazine. The processes of the enzyme catalyzed reaction and the electroreduction of the product of the enzyme catalyzed reaction are described. |
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| Keywords: | o phenylenediamine voltammetry immunoassay enzyme catalyzing horseradish peroxidase |
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