| 单链构象多态性-毛细管电泳分析中样品纯化研究 |
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| 引用本文： | 石先哲,许国旺,赵春霞,杨青.单链构象多态性-毛细管电泳分析中样品纯化研究[J].分析科学学报,2004,20(4):361-363. |
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| 作者姓名： | 石先哲 许国旺 赵春霞 杨青 |
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| 作者单位： | 中国科学院大连化学物理研究所,国家色谱研究分析中心,辽宁,大连,116011 |
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| 基金项目： | 中国科学院知识创新工程领域前沿项目(No.K2001A4) |
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| 摘 要： | 聚合酶链反应(PCR)样品中的Cl^-、引物等小分子对单链构象多态性一毛细管电泳分析有重要影响。本文用毛细管电泳一间接紫外法测定了PCR样品中的Cl^-浓度，并对乙醇沉淀法和试剂盒纯化法用于PCR样品的纯化效果进行比较，在此基础上研究了Cl^-浓度、引物及其二聚体在SSCP-CE分析中的影响。结果表明，它们主要影响DNA进样量而对分离效率影响不大。
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| 关 键 词： | 单链构象多态性 毛细管电泳 PCR样品纯化 |
| 文章编号： | 1006-6144(2004)04-0361-03 |
| 修稿时间： | 2003年3月4日 |
Effect of Sample Purification on Single-strand Conformation Polymorphism Analysis by Capillary Electrophoresis |
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| SHI Xian-zhe,XU Guo-wang,ZHAO Chun-xia,YANG Qing.Effect of Sample Purification on Single-strand Conformation Polymorphism Analysis by Capillary Electrophoresis[J].Journal of Analytical Science,2004,20(4):361-363. |
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| Authors: | SHI Xian-zhe XU Guo-wang ZHAO Chun-xia YANG Qing |
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| Abstract: | Chloride and primers present in the PCR sample have an effect on single-strand conformation polymorphism analysis by capillary electrophoresis using electrokinetic injection. In this paper, the concentration of chloride ion in the PCR sample was quantitatively determined by a CE-indirect UV method. The ethanol precipitation method and PCR sample purified kit for purified PCR samples were compared. The effects of chloride ion concentration and primers on SSCP-CE analysis were investigated. It was found that the amount of DNA fragments injected into the capillary column decreased with the increase of chloride concentration, while the separation efficiency of DNA fragments did not change. |
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| Keywords: | Capillary electrophoresis Single-strand conformation polymorphism PCR sample purification |
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