| 重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究 |
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| 引用本文： | 段昕,张红,邱嵘,高清祥,闵凤玲,郝冀芳,刘兵,周清明,李小达,王燕玲,周光明.重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究[J].原子核物理评论,2007,24(1):55-58. |
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| 作者姓名： | 段昕 张红 邱嵘 高清祥 闵凤玲 郝冀芳 刘兵 周清明 李小达 王燕玲 周光明 |
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| 作者单位： | 1 中国科学院近代物理研究所, 甘肃 兰州 730000;2 中国科学院研究生院, 北京 100049;3 兰州大学生命科学院, 甘肃 兰州 730000 |
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| 基金项目： | 中国科学院"百人计划" |
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| 摘 要： | 探讨12C6+ 离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒（AdCMV GFP）转染小鼠黑色素瘤细胞(B16细胞系)的影响。 采用不同剂量的12C6+ 重离子束辐射经AdCMV GFP 转染的B16细胞， 利用流式细胞仪检测腺病毒的转染率。 结果表明， 12C6＋重离子束辐射能提高腺病毒对B16细胞的转染率， 且具有量效关系。 此外， 先转染后辐射法比起先辐射后转染法能更显著地提高转染率。The effect of 12C6+ beam irradiation on AdCMV GFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMV GFP were irradiated by different doses of 12C6+ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that 12C6+ beam irradiation can improve tansfection efficiency of AdCMV GFP on murine melanoma cell B16 in a dose dependent manner. In addition， the tansfection efficiency in pre tranfection plus irradiation group is higher than that in pre irradiation plus tranfection group at the same dose irradiation dose.
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| 关 键 词： | 12C6+离子束 重组腺病毒载体 转染效率 小鼠黑色素瘤细胞 |
| 文章编号： | 1007-4627（2007）01-0055-04 |
| 收稿时间： | 1900-01-01 |
| 修稿时间： | 2005-06-19 |
Improvement of AdCMV-GFP Gene Transfection Efficiency Induced by Heavy-ion Beam Irradiation on Murine Melanoma Cells |
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| DUAN Xin,ZHANG Hong,QIU Rong,GAO Qing-xiang,MIN Feng-ling,HAO Ji-fang,LIU Bing,ZHOU Qing-ming,LI Xiao-da,WANG Yan-ling,ZHOU Guang-ming.Improvement of AdCMV-GFP Gene Transfection Efficiency Induced by Heavy-ion Beam Irradiation on Murine Melanoma Cells[J].Nuclear Physics Review,2007,24(1):55-58. |
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| Authors: | DUAN Xin ZHANG Hong QIU Rong GAO Qing-xiang MIN Feng-ling HAO Ji-fang LIU Bing ZHOU Qing-ming LI Xiao-da WANG Yan-ling ZHOU Guang-ming |
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| Institution: | 1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China; 3 School of Life Science, Lanzhou University, Lanzhou, 730000, China |
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| Abstract: | The effect of 12C6 beam irradiation on AdCMV-GFP(a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated.B16 cells infected with AdCMV-GFP were irradiated by different doses of 12C6 beam.The transfection efficiency was assessed by flow cytometry(FCM).Results show that 12C6 beam irradiation can improve tansfection efficiency of AdCMV-GFP on murine melanoma cell B16 in a dose-dependent manner.In addition,the tansfection efficiency in pre-tranfection plus irradiation group is higher than that in pre-irradiation plus tranfection group at the same dose irradiation dose. |
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| Keywords: | 12C6 heavy-ion beam AdCMV-GFP transfection efficiency murine melanoma |
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