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重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究
引用本文:段昕,张红,邱嵘,高清祥,闵凤玲,郝冀芳,刘兵,周清明,李小达,王燕玲,周光明.重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究[J].原子核物理评论,2007,24(1):55-58.
作者姓名:段昕  张红  邱嵘  高清祥  闵凤玲  郝冀芳  刘兵  周清明  李小达  王燕玲  周光明
作者单位:1. 中国科学院近代物理研究所,甘肃,兰州,730000;中国科学院研究生院,北京,100049
2. 中国科学院近代物理研究所,甘肃,兰州,730000
3. 兰州大学生命科学院,甘肃,兰州,730000
基金项目:中国科学院"百人计划"
摘    要:探讨12C6 离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒(AdCMV-GFP)转染小鼠黑色素瘤细胞(B16细胞系)的影响。采用不同剂量的12C6 重离子束辐射经AdCMV-GFP转染的B16细胞,利用流式细胞仪检测腺病毒的转染率。结果表明,12C6 重离子束辐射能提高腺病毒对B16细胞的转染率,且具有量效关系。此外,先转染后辐射法比起先辐射后转染法能更显著地提高转染率。

关 键 词:12C6  离子束  重组腺病毒载体  转染效率  小鼠黑色素瘤细胞
文章编号:1007-4627(2007)01-0055-04
修稿时间:2005-06-19

Improvement of AdCMV-GFP Gene Transfection Efficiency Induced by Heavy-ion Beam Irradiation on Murine Melanoma Cells
DUAN Xin,ZHANG Hong,QIU Rong,GAO Qing-xiang,MIN Feng-ling,HAO Ji-fang,LIU Bing,ZHOU Qing-ming,LI Xiao-da,WANG Yan-ling,ZHOU Guang-ming.Improvement of AdCMV-GFP Gene Transfection Efficiency Induced by Heavy-ion Beam Irradiation on Murine Melanoma Cells[J].Nuclear Physics Review,2007,24(1):55-58.
Authors:DUAN Xin  ZHANG Hong  QIU Rong  GAO Qing-xiang  MIN Feng-ling  HAO Ji-fang  LIU Bing  ZHOU Qing-ming  LI Xiao-da  WANG Yan-ling  ZHOU Guang-ming
Institution:1 Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China ; 2 Graduate School of Chinese Academy of Sciences, Beijing 100049, China; 3 School of Life Science, Lanzhou University, Lanzhou, 730000, China
Abstract:The effect of 12C6 beam irradiation on AdCMV-GFP(a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated.B16 cells infected with AdCMV-GFP were irradiated by different doses of 12C6 beam.The transfection efficiency was assessed by flow cytometry(FCM).Results show that 12C6 beam irradiation can improve tansfection efficiency of AdCMV-GFP on murine melanoma cell B16 in a dose-dependent manner.In addition,the tansfection efficiency in pre-tranfection plus irradiation group is higher than that in pre-irradiation plus tranfection group at the same dose irradiation dose.
Keywords:12C6 heavy-ion beam  AdCMV-GFP  transfection efficiency  murine melanoma
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