重离子辐射提高腺病毒介导小鼠黑色素瘤细胞转染率的研究

探讨12C6+ 离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒（AdCMV GFP）转染小鼠黑色素瘤细胞(B16细胞系)的影响。 采用不同剂量的12C6+ 重离子束辐射经AdCMV GFP 转染的B16细胞， 利用流式细胞仪检测腺病毒的转染率。 结果表明， 12C6＋重离子束辐射能提高腺病毒对B16细胞的转染率， 且具有量效关系。 此外， 先转染后辐射法比起先辐射后转染法能更显著地提高转染率。The effect of 12C6+ beam irradiation on AdCMV GFP (a replication deficient recombinant adenoviral vector containing CMV promoter and green fluorescent protein) gene transfection efficiency for murine melanoma cell B16 has been investigated. B16 cells infected with AdCMV GFP were irradiated by different doses of 12C6+ beam. The transfection efficiency was assessed by flow cytometry (FCM). Results show that 12C6+ beam irradiation can improve tansfection efficiency of AdCMV GFP on murine melanoma cell B16 in a dose dependent manner. In addition， the tansfection efficiency in pre tranfection plus irradiation group is higher than that in pre irradiation plus tranfection group at the same dose irradiation dose.     

辐射基因治疗在肿瘤治疗中的研究现状

目前肿瘤基因治疗尚存在许多问题, 距临床应用还有相当的距离, 但是在传统的放疗、化疗和手术治疗的基础上, 辐射与基因治疗的有机结合在肿瘤治疗中却显示出可喜的前景。综述了近年来这一领域的研究进展, 探讨了这一疗法对肿瘤治疗的应用前景。Although tumor gene therapy has a distance to clinical use due to some problems, the combination of irradiation and gene therapy holds much promise in cancer therapy based on the traditional radiotherapy, chemotherapy and surgery. We have termed this therapeutic radiogenic therapy. This review focuses on the group of radiogenic therapy that are either: ⑴ improvement of gene transfer efficiency by irradiation; ⑵ radiotherapy combined with cytokines gene delivery or enhancement of the immunity of tumor cells by transgene; ⑶ directly stimulated by radiation to produce either directly or indirectly cytotoxic agents; ⑷ increasing of radiosensitivity in gene therapy; ⑸ radioprotective gene therapy enhances radiation tumor killing effect while protecting the normal tissue and organs with transgene using transfer vector.     

辐射诱导的DNA修复与细胞凋亡的ATP调控

综述了DNA辐射损伤导致的细胞阻遏于G1期以及在该时期对DNA的修复活动， 提出了较大剂量辐射诱导的三磷酸腺苷不足导致细胞凋亡的假说， 并分析了细胞走向凋亡与修复的辨正关系。 DNA damage induced by irradiation，which makes the cell arrested at G1 stage and DNA repair being activated in this stage，are summarized. It is proposed that the deficiency of adenosine triphosphate which is induced by the larger irradiation dose, induces cell apoptosis. And the relationship of cell selecting repair and apoptosis is also analyzed.