Microscopy of non-birefringent transmissive phase samples using Sagnac laser interferometer |
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| Institution: | 1. Department of Applied Optics and Photonics, University of Calcutta, JD-2, Sector-III, Saltlake, Kolkata 700098, India;2. Department of Electronics and Communication Engineering, Techno India, EM4/1, Saltlake, Kolkata 700091, India;1. Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China;2. Shanghai Laboratory Animal Research Centre, Shanghai Institutes for Biological Sciences, Shanghai, China;1. Wroc?aw University of Technology, Faculty of Microsystem Electronics and Photonics, ul. Z. Janiszewskiego 11/17, PL-50372 Wroc?aw, Poland;2. Wroc?aw University of Technology, Faculty of Mechanical Engineering, ul. I. ?ukasiewicza 5, PL-50371 Wroc?aw, Poland;3. University at Albany, College of Nanoscale Science and Engineering, 257 Fuller Road, Albany, NY 12203, USA |
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| Abstract: | A cyclic interferometer, appropriately combined with a long working distance microscope objective, is adapted for quantitative phase microscopy. In such an arrangement, the sample information, in terms of the diffracted orders emerging from the sample, is carried by both the counter propagating beams within the cyclic interferometer. However, positioning the sample close to the input/output cube beam splitter and use of a suitably converging laser beam of light as the input to the interferometer ensure that only one of the counter propagating beams carries the object information to the objective while the other beam, which serves as the reference, allows only the undiffracted component to contribute to the process of image formation. Use of suitable polarization optics renders the interferometer its polarization phase shifting property. Using the proposed arrangement, the experimental results showing the quantitative 3D phase rendering of polystyrene microspheres and micro-wells etched in glass are presented. |
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| Keywords: | Sagnac interferometer Phase measurement Polarization Microscopy Interference microscopy Polarization phase microscopy |
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