| K562细胞摄取光敏剂ZnPcS2P2的测定及药代动力学 |
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| 引用本文： | 谢宝刚,黄慧芳,黄剑东,陈耐生,黄金陵.K562细胞摄取光敏剂ZnPcS2P2的测定及药代动力学[J].光谱学与光谱分析,2004,24(11):1415-1418. |
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| 作者姓名： | 谢宝刚 黄慧芳 黄剑东 陈耐生 黄金陵 |
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| 作者单位： | 1. 福州大学化学化工学院,福州大学功能材料研究所,福建,福州,350002
2. 福建医科大学附属协和医院血液病研究所,福建,福州,350001 |
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| 基金项目： | 国家自然科学基金青年基金(20201005), 教育部高等学校骨干教师资助计划和福建省自然科学基金(C010005)资助项目 |
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| 摘 要： | 通过荧光分析方法，比较了2．0％SDS(Wt％)，1．5％Triton X-100(φ为体积百分比V／V)及DMF等不同溶剂对K562细胞内摄取的两亲性酞菁锌ZnPcS2P2提取效率，结果表明Triton X-100具有较高的一次提取率。以1．5％Triton X-100为提取剂，获得了K562细胞摄取ZnPcS2P2及ZnPcS2P2-低密度脂蛋白(LDL)复合物的动力学曲线。结果显示，K562细胞对ZnPcS2P2-LDL的最大摄取量是ZnPcS2P2的两倍，表明LDL是有效的靶向载体。
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| 关 键 词： | K562细胞 LDL 摄取量 药代动力学 光敏剂 低密度脂蛋白 SDS X-100 两亲性 荧光分析 |
| 文章编号： | 1000-0593(2004)11-1415-04 |
| 修稿时间： | 2003年7月6日 |
Determination and Pharmacokinetics of Photosensitizer ZnPcS2P2 Uptaken by K562 Cells |
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| XIE Bao gang ,HUANG Hui fang ,HUANG Jian dong ,CHEN Nai sheng ,HUANG Jin ling.Determination and Pharmacokinetics of Photosensitizer ZnPcS2P2 Uptaken by K562 Cells[J].Spectroscopy and Spectral Analysis,2004,24(11):1415-1418. |
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| Authors: | XIE Bao gang HUANG Hui fang HUANG Jian dong CHEN Nai sheng HUANG Jin ling |
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| Institution: | Department of Chemistry, Institute of Research on Functional Materials, Fuzhou University, Fuzhou 350002, China. |
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| Abstract: | The di-sulfonated di-phthalimidomethyl phthalocyanine zinc (ZnPcS2P2) was an amphiphilic photosensitizer for photodynamic therapy of cancer. According to fluorescence analytical method, extracting efficiencies of 2.0% SDS (Wt%), 1.5% Triton X-100 (phi) and DMF for ZnPcS2P2 in K562 cells were compared. The results showed that 1.5% Triton X-100 was the most efficient reagent for the first time extracting. Using 1.5% Triton X-100 as extracting reagent, the kinetic curves of cellular uptake of ZnPcS2P2 as well as LDL-ZnPcS2P2 complex by K562 cells were determined. Compared with ZnPcS2P2, the maximum cellular uptake of LDL-ZnPcS2P2 by K562 was doubled, which indicated that LDL was an effective targeting delivery vehicle for ZnPcS2P2. |
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| Keywords: | Phthalocyanine Cells Fluorescence spectra Kinetics |
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