海洋微藻生长过程藻液三维荧光特征

利用三维荧光光谱技术监测了中肋骨条藻(Skeletonema costatum),塔玛亚历山大藻(Alexandriumtamarense),微小亚历山大藻(Alexandrium mimntum),锥状斯氏藻(Scrippsiella trochoidea),东海原甲藻(Prorocentrum donghaiense)及海洋原甲藻(Prorocentrum micans)实验室培养过程中产生的荧光溶解有机物。中肋骨条藻为代表的硅藻主要产生类腐殖质荧光物质,而甲藻在指数增长期主要产生类蛋白荧光物质。进入消亡期后类蛋白荧光和类腐殖质荧光强度迅速增大,原因可能是衰老、死亡藻细胞的破碎释放出大量的荧光有机物质所致,此外还有细菌对非荧光有机物进一步降解。塔玛亚历山大藻、微小亚历山大藻、东海原甲藻及海洋原甲藻的类蛋白荧光强度在消亡后期由于细菌降解或光降解等因素而降低。同属微藻产生的荧光物质相似,例如塔玛亚历山大藻与微小亚历山藻、东海原甲藻与海洋原甲藻,但具体荧光峰位置有所不同。

Three Dimensional Fluorescence Excitation-Emission Matrix Spectrum of Dissolved Organic Substance in Marine Microalgaes Growth Process

The three-dimensional fluorescence spectrum was used to detect the changes in dissolved organic substances from the cultured Skeletonema costatum,Alexandrium tamarense,Alexandrium mimutum,Scrippsiella trochodea,Prorocentrum donghaiense and Prorocentrum micans.The result indicates that all of the miroalgaes can produce FDOM in the growth courses.Diatom such as Skeletonema costatum can produce humic-like FDOM.However dinoflagellate can produce protein-like FDOM at exponential growth phase.When the algae grows into decadency phase,the intensity of humic-like and protein-like fluorescence augments rapidly,which may be due to a mass of FDOM realeased by the old or dead cell fragmentation and the degradation of bacteria by using non-FDOM.The fluorescent intensity of Alexandrium tamarense,Alexandrium mimutum,Prorocentrum donghaiense and Prorocentrum micans can reduce at anaphase of decadency phase because of the degradation of bacteria and light.The same genus of algae can produce similar FDOM,for example: Alexandrium tamarense,and Alexandrium mimutum,Prorocentrum donghaiense and Prorocentrum micans,but the positions of the fluorescence peaks are different.