水体中溶解有机物激光诱导荧光光谱分析方法

讨论了用激光诱导荧光(LIF)的方法分析水体中溶解有机物(DOM)的含量，利用拉曼散射信号对荧光光谱进行归一化处理，消除激发光强度和水体对荧光的二次吸收和接收条件等因素的影响，并给出了理论依据，由此依据得出了非线性浓度校准的数学模型。利用这个数学模型对实验数据进行非线性拟合，拟合的相关系数高于0.99。该浓度校准的数学模型，考虑到了水体对荧光信号的二次吸收以及溶液浓度的变化对二次吸收的影响，结果表明它可作为不同水体的通用浓度校准的数学模型对水体中的DOM进行定量分析。

An Approach to Analyzing Laser Induced Fluorescence Spectrum of Dissolved Organic Matter

The method of laser induced fluorescence used to analyse dissolved organic matter(DOM) in water was discussed.The fluorescence spectrum was normalized by the intensity of Raman scattering for reducing some negative effect induced by the intensity of excitation,the fluorescence absorption by water,and receiving conditions etc.The fluorescence spectrum normalization theory was deduced,and a nonlinear concentration calibration mathematic model based on the normalization theory was presented.The mathematic model was adopted for nonlinear least-squares data fitting to process experimental data,and a high correlation coefficient excelling 0.99 was obtained.The experimental result indicated that the nonlinear concentration calibration mathematic model based on the consideration of the fluorescence absorption by water and the effect of different concentration of DOM on the absorption was applicable to quantitative analyzing DOM in different water as a general concentration calibration mathematic model.