3-羟基黄酮在不同极性和酸碱度溶剂中的光谱研究

实验观测了3-羟基黄酮(3-HF)在不同极性溶剂中的吸收光谱和荧光光谱，发现在吸收光谱中有3个吸收带，峰值位于300和345 nm的两个吸收带较强，位于415 nm处的吸收带较弱。用345 nm作为激发光，观测到两个荧光带，其中峰值位于400 nm的荧光带为3-HF稀醇式构型的发射，随着溶剂极性的增大其强度增强，峰值位于526 nm的荧光带为3-HF互变异构体的发射，随着溶剂极性的增大其强度减弱，这表明溶剂极性阻碍质子转移的发生。用415 nm的光激发样品，在荧光光谱中发现了3个新荧光谱带，峰值分别位于440，471和515 nm，这3个荧光谱带归属至今未见报道。为了指认这3个荧光谱带，分别观测了3-HF在不同酸碱度溶液的荧光光谱及其吸收光谱，通过对这些光谱的分析研究，指认出荧光峰位于440和471 nm的荧光谱带为3-HF的两种阳离子的发射，峰值位于515 nm的荧光谱带为3-HF的阴离子的发射。

Spectroscopic Investigation of 3-Hydroxyflavone in Different Polarity and pH Values Solutions

The absorption and fluorescence spectra of 3-hydroxyflavone (3-HF) in different polar solvents were observed with UV-Vis spectrometer and fluorescence spectrometer,respectively. There are three absorption bands in the absorption spectra,wherein two absorption bands with absorption peak at 300 and 345 nm,respectively,are strong,and the other one with absorption peak at 415 nm is weak. When the samples in different polar solvents were excited by 345 nm light,there appeared two new fluorescence bands peaked at...