| 单个活态细胞显微激光拉曼散射光谱处理方法研究 |
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| 作者姓名： | Kang LL Huang YX Wu ZJ |
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| 作者单位： | 1. 南方医科大学生物医学工程学院医学工程系,广东,广州,510515
2. 暨南大学生物医学工程研究所,广东,广州,510632 |
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| 基金项目： | 国家自然科学基金项目(30940019,60377043)资助 |
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| 摘 要： | 单细胞激光共焦拉曼散射光谱由于受扫描条件、样品自身浓度以及样品分子组成等因素的影响,其谱线信噪比较低、荧光背景有时较明显从而导致谱线质量较差.本文主要针对活态单细胞的拉曼光谱进行谱线预处理技术研究用以提高谱线的信噪比、灵敏度及降低荧光背景干扰.首先是宇宙射线的去除,之后进行谱线的归类,这是提高谱线灵敏度的关键步骤;其次...
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| 关 键 词： | 显微激光共焦拉曼光谱 单细胞 归类 信噪比 滤波 |
Study of spectrum processing method for Raman microscopy on single living cell |
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| Kang LL,Huang YX,Wu ZJ.Study of spectrum processing method for Raman microscopy on single living cell[J].Spectroscopy and Spectral Analysis,2011,31(2):408-411. |
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| Authors: | Kang Li-Li Huang Yao-Xiong Wu Zheng-Jie |
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| Institution: | KANG Li-li1,HUANG Yao-xiong2,WU Zheng-jie2 1.Department of Biomedical Engineering,Southern Medical University,Guangzhou 510515,China 2.Department of Biomedical Engineering,Jinan University,Guangzhou 510632,China |
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| Abstract: | To study the spectrum processing methods for Raman microscopy on single living cell and develop the pre-process techniques for Raman spectrum of single living cell to enhance the signal to noise ratio,sensitivity,and decrease the fluorescence influence,wiping off the cosmic rays was used to improve the spectrum.The spectra classification,spectra average and filtration were applied to enhance signal to noise ratio.The fluorescence was depressed for quantity analysis or utilized for analysis by comparing the ... |
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| Keywords: | Laser confocal Raman spectroscopy Single living cell Classification Signal to noise ratio Filter |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
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