| 傅里叶红外光谱研究血清白蛋白构象 |
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| 引用本文： | 童义平,李伟,林燕文.傅里叶红外光谱研究血清白蛋白构象[J].光谱学与光谱分析,1999,19(5):704-706. |
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| 作者姓名： | 童义平 李伟 林燕文 |
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| 作者单位： | 韩山师范学院化生系,521041,潮州 |
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| 摘 要： | 用傅里叶红外光谱法研究了ＢＳＡ及其水溶液的红外光谱。通过对其酰胺Ｉ带傅里叶自转积谱分析，为其及部分水溶液中的二级结构构象进行了指认。结果表明，ＢＳＡ水溶液状态与固态时的二级结构是不同的。随着溶液浓度的降低，酰胺Ｉ带二级结构峰存在明显的位移现象，即１６０９．８６ｃｍ＾－１位移到１６０８．２４ｃｍ＾－１，１６３３．８５位移到１６３８．３６ｃｍ＾－１，１６５３．６９ｃｍ＾－１位移到１６５６．１０ｃｍ＾－
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| 关 键 词： | 傅里叶红外光谱 牛血清白蛋白 二级结构 构象 自转积 |
Study of Conformation of Serum Albumin by FTIR |
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| Yiping TONG,Wei LI,Yanwen LIN.Study of Conformation of Serum Albumin by FTIR[J].Spectroscopy and Spectral Analysis,1999,19(5):704-706. |
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| Authors: | Yiping TONG Wei LI Yanwen LIN |
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| Institution: | Department of Bio-Chemistry, Hanshan Teacher's College, 521041 Chaozhou. |
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| Abstract: | This paper used the FTIR method to study the spectra of bovine serum albumin (BSA) and its solution. The secondary structure and conformation was assigned by FTIR-deconvolution analysis. The result indicated that BSA secondary structure in solid is different from in aqueous solution. As the decrease of BSA concentration from 3.241 x 10(-1) to 4.032 X 10(-2) g X mL(-1), the amide I band components at 1 609.84, 1 633.85,1 653.69, 1 681.16 and 1 694.88 cm(-1) shifted to 1 608. 24, 1 638.36, 1 656.10, 1 674.87 and 1 690.78 cm(-1), respectively, the component at 1 621.50 cm(-1) unchanged. |
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| Keywords: | FTIR Bovine serum albumin Secondary structure Conformation Deconvolution |
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