| RP-HPLC测定有边瓦韦中的蜕皮甾酮 |
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| 引用本文： | 张晓静,马骥,朱贞贞,张昊.RP-HPLC测定有边瓦韦中的蜕皮甾酮[J].光谱实验室,2012,29(3):1834-1837. |
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| 作者姓名： | 张晓静 马骥 朱贞贞 张昊 |
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| 作者单位： | 陕西师范大学生命科学学院西北濒危药材资源开发国家工程实验室 西安市长安南路199号 710062 |
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| 基金项目： | 国家“十一五”科技支撑计划 |
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| 摘 要： | 采用色谱柱Shimadzu C18(250mm×4.6mm,5μm),流动相为甲醇-水(49:51,V/V),流速为0.8mL/min,检测波长为324nm,柱温为30℃,建立测定有边瓦韦中蜕皮甾酮含量的反相高效液相色谱法。结果为蜕皮甾酮在0.11—1.65μg范围内线性关系良好(R2=0.9991),平均回收率为103.4%,RSD为1.53%。本方法简便准确、灵敏度高、重复性好,可用于有边瓦韦中蜕皮甾酮的含量测定。
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| 关 键 词： | 有边瓦韦 蜕皮甾酮 反相高效液相色谱法 |
Determination of Ecdysterone in Lepisorus marginatus Ching by RP-HPLC |
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| ZHANG Xiao-Jing
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MA Ji
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ZHU Zhen-Zhen
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ZHANG Hao.Determination of Ecdysterone in Lepisorus marginatus Ching by RP-HPLC[J].Chinese Journal of Spectroscopy Laboratory,2012,29(3):1834-1837. |
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| Authors: | ZHANG Xiao-Jing MA Ji ZHU Zhen-Zhen ZHANG Hao |
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| Institution: | (College of Life Sciences,Shaanxi Normal University,National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China,Xi’an 710062,P.R.China) |
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| Abstract: | A method for the determination of ecdysterone in Lepisorus marginatus Ching by RP-HPLC was established.The Shimadzu C18(250mm×4.6mm,5μm) was used for analysis,with detection wavelength 324nm,flow rate 0.8mL/min,column temperature 30℃ and methanol-H2O(49∶51) as mobile phase.A good linear curve in the range of 0.11—1.65μg(R2=0.9991) was obtained.The average recovery was 103.4% with a relative standard deviation(RSD)of 1.53%.The method is simple and accurate,sensitive and reproducible,and can be effectively applied for the determination. |
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| Keywords: | Lepisorus marginatus Ching Ecdysterone RP-HPLC |
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