| Folin-Ciocalteu比色法测定石榴不同部位总多酚的含量 |
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| 引用本文： | 田树革,魏玉龙,刘宏炳.Folin-Ciocalteu比色法测定石榴不同部位总多酚的含量[J].光谱实验室,2009,26(2):341-344. |
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| 作者姓名： | 田树革 魏玉龙 刘宏炳 |
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| 作者单位： | 1. 新疆医科大学中医学院中药系,乌鲁木齐市新医路393号,830011
2. 新疆伊犁州中医院药剂室,新疆伊犁市,835000 |
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| 基金项目： | 新疆维吾尔自治区科技平台项目 |
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| 摘 要： | 石榴含有丰富的多酚类化合物（含量因部位不同而有差异）,这类化合物具有抗动脉硬化、降低胆固醇、抗氧化和抗辐射等作用,可用来评价石榴的活性。本文采用Folin-Ciocalteu法对石榴中的总多酚进行检测。以没食子酸为对照品,校准曲线的回归方程为y=0.04659＋109.89x（r=0.9994）;对稳定性、精密度、重现性、回收率进行了试验,该方法简便、快速、准确,是检测石榴中总多酚的可靠方法。
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| 关 键 词： | 石榴籽 石榴皮 石榴汁 总多酚 分光光度法 |
Determination of Total Polyphenol in the Different Places of Punica Granatum L by Folin-Ciocalteu Colorimetry |
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| TIAN Shu-Ge,WEI Yu-Long,LIU Hong-Bing.Determination of Total Polyphenol in the Different Places of Punica Granatum L by Folin-Ciocalteu Colorimetry[J].Chinese Journal of Spectroscopy Laboratory,2009,26(2):341-344. |
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| Authors: | TIAN Shu-Ge WEI Yu-Long LIU Hong-Bing |
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| Institution: | College of TCM;Xinjiang Medical University;Urumqi 830054;P.R.China;Traditional Chinese Medical Hospital of Xinjiang Yili Region;Yili;Xinjiang 835000;P.R.China |
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| Abstract: | There are rich polyphenolic compounds in the different places of Punica granatum L,which have multi-functions,such as anti-arteriosclerosis,cholesterol lowering function,anti-oxidation and anti-radiation.The polyphenolic compounds could be evaluated as theactivities of Punica granatum L. Total polyphenolic compounds in the different parts of Punica granatum L were determined by Folin-Ciocalteu method.The standard reference is gallic acid and the regressive equation of standard curve is y=0.04659+109.89x(r=0... |
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| Keywords: | Punica Granatum L Seed Punica Granatum L Peel Punica Granatum L Juice Total Polyphenolic Spectrophotometry |
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