| 荧光标记微管的光敏断裂及机理 |
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| 引用本文： | 徐春华,刘春香,郭红莲,李兆霖,降雨强,张道中,袁明.荧光标记微管的光敏断裂及机理[J].物理学报,2006,55(1):206-210. |
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| 作者姓名： | 徐春华 刘春香 郭红莲 李兆霖 降雨强 张道中 袁明 |
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| 作者单位： | (1)中国科学院物理研究所,凝聚态物理中心光物理实验室,北京 100080; (2)中国农业大学生物学院植物生理学与生物化学国家重点实验室,北京 100094 |
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| 基金项目： | 中国科学院资助项目;国家重点基础研究发展计划(973计划) |
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| 摘 要： | 荧光标记的微管经激发光照射会引起微管的断裂，但其断裂过程及机理并不清楚.通过光镊研究了微管光敏断裂的动态过程.实验结果表明组成微管的原丝并不是同时断裂的，而是相继断裂最终导致微管的断裂.经过一系列生物学实验，确定了活性氧中的自由基是造成微管光敏断裂的主要因素.根据光镊研究的结果推测了自由基作用于微管的机理.
关键词：
光镊
微管
断裂
自由基
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| 关 键 词： | 光镊 微管 断裂 自由基 |
| 文章编号： | 1000-3290/2006/55(01)/0206-05 |
| 收稿时间： | 03 24 2005 12:00AM |
| 修稿时间： | 2005-03-242005-05-11 |
Photosensitive breaking of fluorescent labeled microtubules and its mechanism |
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| Xu Chun-Hua,Liu Chun-Xiang,Guo Hong-Lian,Li Zhao-Lin,Jiang Yu-Qiang,Zhang Dao-Zhong,Yuan Ming.Photosensitive breaking of fluorescent labeled microtubules and its mechanism[J].Acta Physica Sinica,2006,55(1):206-210. |
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| Authors: | Xu Chun-Hua Liu Chun-Xiang Guo Hong-Lian Li Zhao-Lin Jiang Yu-Qiang Zhang Dao-Zhong Yuan Ming |
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| Institution: | 1 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China; 2 Optical Physics Laboratory, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | It is reported that fluorescent microtubules may break under irradiation. However, the process and mechanism are not well understood. In this paper, we study the process of light-induced microtubule breaking by optical tweezers and find the breakup of protofilaments in microtubule is not simultaneous but proceeds step-by-step. After a series of biological experiments, we confirm that free radicals are the main reason for the photosensitive breaking of microtubule. According to the results of optical tweezers experiments, the mechanism of free radicals acting on microtubule is infered. |
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| Keywords: | optical tweezers microtubule break free radicals |
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