延龄草中2 个甾体皂苷的2D NMR 研究

采用柱色谱法从延龄草中分离得到两个甾体皂苷化合物，即(25S)-27-羟基-偏诺皂苷元-3β-O-α-L-吡喃鼠李糖基-(1→4)-O-α-L-吡喃鼠李糖基-(1→2)]-O-β-D-吡喃葡萄糖苷{(25S)-27-hydroxy-penogenin-3β-O-α-L-rhamnopyranosyl-(1→4)-O-α-L-rhamnopyranosyl-(1→2)]-O-β-D-glucopyranoside, 1} 、(25S)-27- 羟基- 偏诺皂苷元-3β-O-α-L- 吡喃鼠李糖基-(1→4)-O-α-L-吡喃鼠李糖基-(1→4)-O-α-L-吡喃鼠李糖基-(1→2)]-O-β-D-吡喃葡萄糖苷(polyphylloside III, 2)，应用¹H NMR，¹³C NMR，DEPT，HSQC，HMBC，¹H-¹H COSY 和NOESY NMR 技术进行结构解析，并对化合物1，2 的¹H NMR，¹³C NMR 数据进行全归属．

A Two-Dimensional NMR Study on Steroid Glycosides Extracted from Trillium tschonoskii

(25S)-27-hydroxypenogenin-3β-O-α-L-rhamnopyranosyl-(1→4)-O-α-L-rhamno-pyranosyl-(1→2)]-O-β-D-glucopyranoside (1) and polyphylloside III (2) were isolated from Trillium tschonoskii by column chromatography. One-dimensional and two-dimensional NMR techniques, including 1H NMR, 13C NMR, DEPT, HSQC, HMBC and 1H-1H COSY, were used to elucidate the structures of these compounds. 13C NMR and 1H NMR chemical shifts of the two compounds were completely assigned.