新型锌离子荧光探针(5-(二甲基氨基)-N-(4-(2-(2-喹啉亚甲基)甲酰肼基)苯基)萘-1-磺酰胺)的研究

以丹磺酰胺为荧光基团设计合成了新型Zn²⁺荧光探针DW1(5-(二甲基氨基)-N-(4-(2-(2-喹啉亚甲基)甲酰肼基)苯基)萘-1-磺酰胺)。通过紫外光谱、荧光光谱及电喷雾质谱研究了DW1对Zn²⁺的选择性识别作用。结果表明,DW1与Zn²⁺结合后荧光显著增强,荧光发射光谱由545 nm蓝移至515 nm,量子产率达到0.32,且对Zn²⁺具有较高的选择性,受常见离子的干扰较小。光谱滴定和ESI-MS谱表明DW1与Zn²⁺以1∶1的化学计量数形成配合物,平衡常数K=1.75×10⁴(mol/L)^-1。

Synthesis of(E)-5-(dimethylamino)-N-(4-(2-(quinolin-2-ylmethylene)-hydrazinecarbonyl)phenyl) naphthalene-1-sulfonamide for Detection of Zinc Ion

A novel dansyl sulfonamide-based fluorescence probe,(E)-5-(dimethylamino)-N-(4-(2-(quinolin-2-ylmethylene)hydrazinecarbonyl)phenyl)naphthalene-1-sulfonamide(DW1) was synthesized for detection of Zn2+.UV-Vis,fluorescence and ESI-MS spectra of DW1 were measured to investigate the selective recognition.The results show that the fluorescence intensity increase significantly with high quantum yield(Φf=0.32) and blue-shift emission from 545 nm to 515 nm after the coordination of DW1 with Zn2+,manifesting DW1 could detect Zn2+ sensitively.Moreover,the selective and competitive fluorescent experiments show that DW1 is highly selective for recognition of Zn2+ over other common metal ions.Spectrophotometric titration and ESI-MS spectrum indicate the formation of a 1∶1 DW1-Zn2+ complex and the association constant K was calculated as 1.75×104(mol/L)-1.