毛细管电泳分离和激光检测分析多糖胶

将多糖胶的混合物与荧光剂9-氨基芘-1,4,6-三磺酸(APTS)派生后再进行微量离心过滤分离。所得到的高分子部分采用毛细管电泳(CE)分离和激光诱导荧光(LIF)检测技术进行分析。缓冲溶液pH的调节和聚丙烯酰胺(PAA)涂层毛细管的使用有效地改善了多糖胶的分离效率和峰形。在优化条件下,iota、kappa角叉菜胶、藻胶、xanthan、carboxymethyl cellulose (CMC)等5种组分的混合物和阿拉伯树胶、刺梧桐树胶、CMC等3种组分的混合物分别在pH 3.2和7.8的缓冲溶液下得到了

Analysis of Gums by Capillary Electrophoresis with Laser Induced Fluorescence

Gums were derivatised with the fluorescence reagent, 9-aminopyrene-1,4,6-trisulfonic acid followed by microcentrifuge filtration. The resulting high mass fractions were analysed by capillary electrophoresis (CE) on a polyacrylamide coated capillary with laser induced fluorescence (LIF) detection. A wide pH range of electrolytes was used to study the influences on the electrophoretic mobilities and on the peak shapes of the gums. In this way, the separation of a mixture of five commercial gums, namely iota carrageenan, kappa carrageenan, alginic acid, xanthan and carboxymethyl cellulose (CMC), could be achieved at pH 3.2 with a 25 mmol/L trisodium citrate buffer. It is also shown that a mixture of Arabic gum, Karaya gum and CMC could be separated at pH 7.8 in a similar buffer.