| 茶叶中单宁的荧光动力学分析法研究 |
| |
| 引用本文： | 冯素玲,汤俊明,樊静.茶叶中单宁的荧光动力学分析法研究[J].光谱学与光谱分析,2003,23(2):322-324. |
| |
| 作者姓名： | 冯素玲 汤俊明 樊静 |
| |
| 作者单位： | 河南师范大学化学与环境科学学院,河南省高等学校环境科学与工程重点学科开放实验室,河南,新乡,453002 |
| |
| 基金项目： | 河南省教委自然科学基金(No.1999150030)，河南省高等学校环境科学与工程重点学科开放实验室资助项目 |
| |
| 摘 要： | 本文根据在Britton-Robinson缓冲溶液中单宁对Cu(Ⅱ)催化过氧化氢氧化吡咯红Y具有活化作用,使体系荧光狡灭,建立了一个高灵敏度荧光动力学测定痕量单宁的新方法。详细研究了体系的最佳实验条件,采用固定时间法测定体系的荧光强度。该方法的线性范围为0.06～0.96mg·L~(-1),检出限为0.032mg·L~(-1)。对含量为0.32mg·L~(-1)的单宁标准溶液进行11次平行测定,其相对标准偏差为2.3％。将方法用于茶叶中单宁含量的测定,并与Folin-Ciocalteu光度法作了对照,结果满意。
|
| 关 键 词： | 荧光动力学法 单宁 吡咯红Y 茶叶 |
| 文章编号： | 1000-0593(2003)02-0322-03 |
| 修稿时间： | 2002年1月17日 |
Study on the Kinetic Fluorimetric Determination of Tannins in Tea |
| |
| FENG Su-ling,TANG Jun-ming and FAN Jing
College of Chemistry and Environmental Science,Key Laboratory of Environmental Science and Engineering,Education
Commission of Henan,Henan Normal University,Xinxiang ,China.Study on the Kinetic Fluorimetric Determination of Tannins in Tea[J].Spectroscopy and Spectral Analysis,2003,23(2):322-324. |
| |
| Authors: | FENG Su-ling TANG Jun-ming and FAN Jing
College of Chemistry and Environmental Science Key Laboratory of Environmental Science and Engineering Education
Commission of Henan Henan Normal University Xinxiang China |
| |
| Institution: | College of Chemistry and Environmental Science, Key Laboratory of Environmental Science and Engineering, Education Commission of Henan, Henan Normal University, Xinxiang 453002, China. |
| |
| Abstract: | A simple and highly sensitive kinetic fluorimetric method is proposed for the determination of trace tannins, based on the activation of tannins on the oxidation of pyronine Y by hydrogen peroxide catalyzed by Cu(II) ion. The effects of some experimental conditions were investigated and discussed in detail. The fixed reaction time procedure was used to determine the fluorescence intensity of the system. The calibration curve of tannin was linear in the range of 0.06-0.96 mg.L-1, and the detection limit for tannin was 0.032 mg.L-1. The relative standard deviation for the measurement of 0.32 mg.L-1 tannin (n = 11) was 2.3%. The proposed method has been successfully applied to the determination of tannins in tea. The results obtained were compared with those provided by the Folin-Ciocalteu method. |
| |
| Keywords: | Kinetic fluorimetric method Tannins Pyronine Y Tea |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
