| 毛细管电泳有效分离测定甜菊糖苷新方法的研究 |
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| 引用本文： | 邵寒娟,胡涌刚,丁亮,陈睦传,彭兴跃.毛细管电泳有效分离测定甜菊糖苷新方法的研究[J].分析科学学报,2001,17(2):127-130. |
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| 作者姓名： | 邵寒娟 胡涌刚 丁亮 陈睦传 彭兴跃 |
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| 作者单位： | [1]厦门大学生命科学院生物学与肿瘤工程教育部重点实验室 [2]厦门大学生命科学院生物学与肿 |
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| 基金项目： | 高等学校重点实验室访问学者基金 |
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| 摘 要： | 本文采用100％二甲基甲酰胺（DMF）作为样品的溶剂，以Tris－硼砂作为分离甜菊糖苷的缓冲体系，探讨了进样量、柱温、电压、Tris-硼砂缓冲液浓度以及pH值等条件对甜菊糖苷分离结果的影响。通过优化各种分离条件，实现了毛细管电泳高效分离测定甜菊糖苷。
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| 关 键 词： | 毛细管电泳 分离 检测 二甲基甲酰胺 甜菊 糖苷 |
| 文章编号： | 1006-6144(2001)02-0127-04 |
| 修稿时间： | 2000年7月21日 |
Study of New Method for Separation and Determination of Stevia Glucosides by Capillary Electrophoresis |
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| SHAO Han-juan,HU Yong-Gang,DING Liang,CHEN Mu-zhuan,PENG Xing-yue.Study of New Method for Separation and Determination of Stevia Glucosides by Capillary Electrophoresis[J].Journal of Analytical Science,2001,17(2):127-130. |
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| Authors: | SHAO Han-juan HU Yong-Gang DING Liang CHEN Mu-zhuan PENG Xing-yue |
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| Abstract: | This paper reported a new, effective and secure method for separation and determination of stevioside by capillary electrophoresis. The effects of different separation conditions on the migration time, selectivity and resolution were investigated. Under optimum separation conditions, the detector response for stevioside was linear over the range 0.05-9 mg/ml and the linear regression coefficient was 0.997. All components provided good resolution in 3.5 min. The relative standard deviations for the migration time of St and RA were 0.71% and 0.46%, respectively. This method was applied to the determination of steviol extract, and the content of St in this sample was 5.36%. |
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| Keywords: | CE Stevia Glucosides Separation and determination DMF |
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