| 弱酸树脂D113对S-腺苷-L-蛋氨酸的交换行为及分离应用研究 |
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| 引用本文： | 刘惠,林建平,岑沛霖.弱酸树脂D113对S-腺苷-L-蛋氨酸的交换行为及分离应用研究[J].离子交换与吸附,2003,19(2):104-110. |
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| 作者姓名： | 刘惠 林建平 岑沛霖 |
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| 作者单位： | 浙江大学(玉泉)材化学院生物工程研究所,杭州,310027 |
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| 摘 要： | 考察了S-腺苷-L-蛋氨酸 (S-adenosyl-L-methionine, SAM) 在弱酸树脂争光D113上的静态吸附及分离行为,利用Langmuir等温式对平衡等温线进行了拟合,实验表明,随着pH的提高,平衡交换量增加,提高温度和SAM的初始浓度在一定程度上能促进内扩散过程,利用D113树脂在f 1.7×30.0cm小型柱上从酵母提取液中分离SAM的得率为90%以上,纯度达87%以上。
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| 关 键 词： | S－腺苷－L－蛋氨酸 弱酸树脂 离子交换 酵母 分离 抗抑郁药 |
| 文章编号： | 1001-5493(2003)02-0104-07 |
| 修稿时间： | 2002年8月18日 |
STUDY ON THE ION-EXCHANGE OF S-ADENOSYL-L-METHIONINE ON D113 WEAK ACID RESIN AND ITS APPLICATION ON SEPARATING S-ADENOSYL-L-METHIONINE FROM YEAST CELLS |
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| LIU Hui LIN Jianping CEN Peilin Institute of Bioengineering,College of Materials Science and Chemical Engineering,Zhejiang University,Hangzhou.STUDY ON THE ION-EXCHANGE OF S-ADENOSYL-L-METHIONINE ON D113 WEAK ACID RESIN AND ITS APPLICATION ON SEPARATING S-ADENOSYL-L-METHIONINE FROM YEAST CELLS[J].Ion Exchange and Adsorption,2003,19(2):104-110. |
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| Authors: | LIU Hui LIN Jianping CEN Peilin Institute of Bioengineering College of Materials Science and Chemical Engineering Zhejiang University Hangzhou |
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| Institution: | LIU Hui LIN Jianping CEN Peilin Institute of Bioengineering,College of Materials Science and Chemical Engineering,Zhejiang University,Hangzhou,310027 |
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| Abstract: | |
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| Keywords: | S-Adenosyl-L-methionine (SAM SAM-e AdoMet) Weak acid resin Ion exchange Yeast Separation |
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