| 基于纳米金／壳聚糖掺杂碳纳米管修饰金电极非标记免疫传感器的研究 |
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| 引用本文： | 罗启枚,王辉宪,徐新宇,李彦涛,黄俊,刘登友.基于纳米金／壳聚糖掺杂碳纳米管修饰金电极非标记免疫传感器的研究[J].分析测试学报,2011,30(7):780-783. |
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| 作者姓名： | 罗启枚 王辉宪 徐新宇 李彦涛 黄俊 刘登友 |
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| 作者单位： | 1. 湖南农业大学理学院应用化学系,湖南长沙,410128
2. 湖南农业大学东方科技学院,湖南长沙,410128 |
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| 基金项目： | 湖南农业大学青年基金资助项目（08QN10）；湖南省科技厅计划资助项目（2009NK3107） |
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| 摘 要： | 应用吸附法将羊抗人IgG抗体直接固定于纳米金(GNPs)/壳聚糖(Chit)掺杂碳纳米管(CNTs)修饰的金电极表面,制备了用于人IgG抗原检测的非标记电化学免疫传感器.利用循环伏安法和交流阻抗研究了修饰电极表面的电化学特性,用差分脉冲伏安法研究了测试底液的pH值对免疫传感器性能的影响.实验表明,在含不同浓度人IgG的...
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| 关 键 词： | 壳聚糖 碳纳米管 纳米金 非标记 抗体 人IgG 免疫传感器 |
Study on Label-free Immunosensor Based on Gold Nanoparticles/Chitosan Incorporating Carbon Nanotubes Composite Membrane Modified Gold Electrode |
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| LUOQi-mei,WANGHui-xian,XUXin-yu,LIYan-tao,HUANGJun,LIUDeng-you.Study on Label-free Immunosensor Based on Gold Nanoparticles/Chitosan Incorporating Carbon Nanotubes Composite Membrane Modified Gold Electrode[J].Journal of Instrumental Analysis,2011,30(7):780-783. |
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| Authors: | LUOQi-mei WANGHui-xian XUXin-yu LIYan-tao HUANGJun LIUDeng-you |
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| Institution: | LUO Qi-mei1,WANG Hui-xian1,XU Xin-yu2,LI Yan-tao2,HUANG Jun2,LIU Deng-you1 (1.Department of Applied Chemsitry,Science College,Hunan Agricultural University,Changsha 410128,China,2.Oriental Science and Technology College,China) |
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| Abstract: | A highly sensitive label-free electrochemical immunosensor was developed for the determination of human IgG based on a gold electrode modified with GNPs and carbon nanotubes(CNTs) incorporated with chitosan composite and the IgG antibody was directly absorbed on the modified electrode surface.The electrochemical behaviors of the modified electrode were investigated by cyclic voltammetry(CV) and AC impedance.The influence of electrolyte pH on immunosensor was studied by differential pulse voltammetry(DPV).A ... |
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| Keywords: | glutathione nano-TiO2 caffeic acid modified carbon paste electrode electrocatalytic oxidation electrochemical determination |
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