| 乙肝解毒胶囊中5种有效成分的胶束电动色谱-质谱联用法测定 |
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| 引用本文： | 曾永芳,黄丽涵,徐远金.乙肝解毒胶囊中5种有效成分的胶束电动色谱-质谱联用法测定[J].分析测试学报,2010,29(3). |
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| 作者姓名： | 曾永芳 黄丽涵 徐远金 |
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| 作者单位： | 1. 广西亚热带生物资源保护利用重点实验室,广西,南宁,530004;广西大学糖业工程技术研究中心,广西,南宁,530004
2. 广西亚热带生物资源保护利用重点实验室,广西,南宁,530004 |
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| 基金项目： | 国家自然科学基金资助项目，广西自然科学基金资助项目 |
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| 摘 要： | 建立了胶束电动色谱-电喷雾电离质谱联用法同时测定乙肝解毒胶囊中的小檗碱、巴马汀、药根碱、黄芩苷、大黄素5种药效成分含量的分析方法。使用未涂层石英毛细管,以30 mmol/L月桂酸-70mmol/L氨水溶液(含20%乙腈,pH10.0)为缓冲液、70%异丙醇(含3 mmol/L乙酸)为鞘液。结果表明,在18 min内各组分达到基线分离,小檗碱、巴马汀、药根碱、黄芩苷、大黄素的线性范围分别为0.02~20、0.05~15、0.05~10、2.0~500、0.02~15 mg/L,检出限分别为0.007、0.02、0.02、0.60、0.006 mg/L。样品的加标回收率为95%~104%,相对标准偏差均小于3.9%。方法简便、快速、灵敏,已成功用于乙肝解毒胶囊中小檗碱、巴马汀、药根碱、黄芩苷、大黄素含量的同时测定。
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| 关 键 词： | 胶束电动色谱 电喷雾电离质谱 乙肝解毒胶囊 有效成分 |
Simultaneous Determination of Five Effective Components in Yiganjiedu Capsule by Micellar Electrokinetic Chromatography-Electrospray Ionization Mass Spectrometry |
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| ZENG Yong-fang,HUANG Li-han,XU Yuan-jin.Simultaneous Determination of Five Effective Components in Yiganjiedu Capsule by Micellar Electrokinetic Chromatography-Electrospray Ionization Mass Spectrometry[J].Journal of Instrumental Analysis,2010,29(3). |
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| Authors: | ZENG Yong-fang HUANG Li-han XU Yuan-jin |
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| Institution: | ZENG Yong-fang1,2,HUANG Li-han1,XU Yuan-jin1,2(1.Guangxi Key Laboratory of Subtropical Bio-resource Conservation , Utilization,Nanning 530004,China,2.Center for Sugar Technology Research,Guangxi University,China) |
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| Abstract: | A new method for the simultaneous determination of berberine,palmatine,jatrorrhizine,baicalin and emodin in Yiganjiedu capsule was established by micellar electrokinetic chromatography-electrospray ionization mass spectrometric(MEKC-ESI MS) method.The samples were separated by an uncoated capillary on the operating voltage of 25 kV.The effect of experimental conditions,such as concentration of lauric acid,selection of bases,addition of organic solvent,capillary temperature,applied voltage and the compositio... |
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| Keywords: | micellar electrokinetic chromatography electrospray ionization mass spectrometry Yiganjiedu capsule effective component |
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