4-氨基吡啶印迹聚合物毛细管整体柱的电色谱识别机理

在石英毛细管中原位聚合制备了4-氨基吡啶印迹聚合物毛细管整体柱，通过考察流动相中乙腈比例对4-氨基吡啶、2-氨基吡啶和硫脲在分子印迹聚合物毛细管整体柱、空白聚合物毛细管整体柱和硅烷化衍生的开管柱上迁移时间和分离情况的影响，研究了4-氨基吡啶分子印迹聚合物毛细管整体柱的CEC识别机理。发现有机添加剂的含量对印迹聚合物的印迹识别能力影响很大，甚至能改变混合物的流出顺序。根据随乙腈含量改变混合物迁移时间和流出顺序的变化规律，可以推测：随着乙腈含量的提高，色谱保留对迁移的影响越来越大；随着乙腈含量的降低，电泳对迁移的影响越来越大。

The recognition mechanism of 4-aminopyridine molecularly imprinted polymer-based monolithic capillary column in electrochromatography

aminopyridine molecularly imprinted polymer based monolithic capillary column was prepared by a in situ polymerization process in fused silica capillary column. Based on the comparison of the influence of acetonitrile content on the migration behavior of 4 aminopyridine and 2 aminopyridine on non imprinted polymer based column and silanized open tubular column, the mechanism of the separation on the 4 aminopyridine molecularly imprinted polymer based column by capillary electrochromatography was discussed. The recognition ability of the 4 aminopyridine molecular imprinted polymer was affected by the acetonitrile content so strongly that the elution order of the solutes might be reversed. When the acetonitrile content was high, the chromatographic retention played a main role in the migration process; when the concentration of acetonitrile was low, electrophoretic migration played a main role.