金纳米球/氮掺杂石墨烯量子点固载Ru(bpy)3^2+电致化学发光法检测邻苯二酚

利用还原法制得金纳米球(Au NPs),与氮掺杂石墨烯量子点(NG QDs)杂化后,再以壳聚糖胶黏剂并通过静电作用使Ru(bpy)3^2+负载于其表面形成复合膜,制备了一种新型的固相电致化学发光(ECL)传感器。研究发现,与单一材料相比,Au NPs和NG QDs杂化复合材料作为载体显著提高了发光试剂Ru(bpy)3^2+的ECL信号。根据环境污染物邻苯二酚对该修饰电极ECL信号较强的阻抑作用,建立了测定领苯二酚的新体系。结果表明,Au NPs/NG QDs/Ru(bpy)3^2+修饰电极的ECL信号变化值与邻苯二酚的浓度负对数在5.0 nmol/L^10μmol/L之间呈良好的线性关系,检出限为3.0 nmol/L(r=0.9992)。对5.0μmol/L的邻苯二酚进行10次重复测定,相对标准偏差为4.6%,常见的共存物质不干扰测定,表明该方法的选择性较好。采用该修饰电极成功测定了河水中的邻苯二酚含量。

Au nanospheres/nitrogen-doped graphene quantum dots containing immobilized Ru(bpy)3^2+ for electrochemiluminescence sensing of catechol

Au nanospheres were first prepared via a simple reduction method.After incorporation with nitrogen-doped graphene quantum dots,the product was covered by Ru(bpy)3^2+ through chitosan adhesive to form a composite membrane,which was further utilized to prepare a novel solid phase electrochemiluminescence sensor(AuNPs/NG QDs/Ru(bpy)32+).Comparing with single materials,the incorporation materials of NGQDs and AuNPs effectively enhanced the ECL signal of the Ru(bpy)3^2+.Based on the environmental pollutant of catechol which was able to inhibit the ECL signal of the sensor modified by Au NPs/NG QDs/Ru(bpy)3^2+,a sensitive determination method for catechol was established.The assay displayed a satisfied linear response between the ECL signals and the negative logarithm of the catechol concentrations in the range of 5.0nmol/L-10μmol/L,and the detection limit was 3.0nmol/L(r=0.9992).Repeating 10 measurements for the 5.0μmol/L catechol,the relative standard deviation(RSD)was calculated as 4.6%.The further assays additionally proved that the influence of the common coexisting substances on the determination was limited,indicating the method owned high selectivity.This sensor was then employed to measure the catechol amount in rivers.