Low-parachor solvents extraction and thermostated micro-thin-layer chromatography separation for fast screening and classification of spirulina from pharmaceutical formulations and food samples |
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Authors: | Zarzycki Paweł K Zarzycka Magdalena B Clifton Vicki L Adamski Jerzy Głód Bronisław K |
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Institution: | 1. Section of Toxicology and Bioanalytics, Department of Civil and Environmental Engineering, Koszalin University of Technology, ?niadeckich 2, 75-453 Koszalin, Poland;2. The Robinson Institute, School of Paediatrics and Reproductive Health, Lyell MCEwin Hospital, University of Adelaide, Haydown Rd, Elizabethvale, 5112 SA, Australia;3. Helmholtz Zentrum Muenchen, Institute of Experimental Genetics, Genome Analysis Center, Ingolstaedter Landstr. 1, 85764 Neuherberg, Germany;4. Department of Analytical Chemistry, Institute of Chemistry, Faculty of Science, University of Podlasie, 3 Maja 54, 08-110 Siedlce, Poland |
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Abstract: | The goal of this paper is to demonstrate the separation and detection capability of eco-friendly micro-TLC technique for the classification of spirulina and selected herbs from pharmaceutical and food products. Target compounds were extracted using relatively low-parachor liquids. A number of the spirulina samples which originated from pharmaceutical formulations and food products, were isolated using a simple one step extraction with small volume of methanol, acetone or tetrahydrofuran. Herb samples rich in chlorophyll dyes were analyzed as reference materials. Quantitative data derived from micro-plates under visible light conditions and after iodine staining were explored using chemometrics tools including cluster analysis and principal components analysis. Using this method we could easily distinguish genuine spirulina and non-spirulina samples as well as fresh from expired commercial products and furthermore, we could identify some biodegradation peaks appearing on micro-TLC profiles. This methodology can be applied as a fast screening or fingerprinting tool for the classification of genuine spirulina and herb samples and in particular may be used commercially for the rapid quality control screening of products. Furthermore, this approach allows low-cost fractionation of target substances including cyanobacteria pigments in raw biological or environmental samples for preliminary chemotaxonomic investigations. Due to the low consumption of the mobile phase (usually less than 1 mL per run), this method can be considered as environmentally friendly analytical tool, which may be an alternative for fingerprinting protocols based on HPLC machines and simple separation systems involving planar micro-fluidic or micro-chip devices. |
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Keywords: | Spirulina Herbs Thermostated micro-TLC Reversed-phase planar chromatography Parachor Extraction Multivariate statistic Fingerprinting Drug stability Metabolomics |
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