| Protein Adsorption onto Nanosized Hydroxyapatite Particles for Controlled Drug Release |
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| 作者姓名： | WANG Bo TENG Li-rong WANG Chun-yan MENG Qing-fan ZHAO Ling-zhi GAO Bo |
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| 作者单位： | WANG Bo1，TENG Li-rong1，WANG Chun-yan2，MENG Qing-fan1，ZHAO Ling-zhi1 and GAO Bo11. College of Life Science，2. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry，Jilin University，Changchun 130012，P. R. China |
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| 基金项目： | 吉林大学校科研和教改项目 |
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| 摘 要： | Nanosized hydroxyapatite(nsHAp) was synthesized to examine its possibility as a controlled release carrier of protein. To achieve effective protein release from nanosized hydroxyapatite, the study of the adsorptive properties of protein on nsHAp and different influence parameters such as pH, calcium, and phosphate concentrations during the adsorption process is necessary. Ovalbumin(OVA) was selected as the model of growth factors. The results show that the amount of OVA adsorbed onto nsHAp in acetic buffer(pH=3.6) is more than that in acetic buffer(pH=5.6) because of the electric interaction. The amount of OVA adsorption in phosphate buffer solution(PBS) is smaller than that in acetic buffer because of surface complexation and surface hydroxylation. The presence of Ca2 dramatically increases the adsorbed amount of OVA in acetic buffer on maintaining the same pH. Meanwhile, the release kinetics of OVA adsorbed onto nsHAp(nsHAp-OVA) was also examined. The amount of released OVA in PBS(pH=5.6) was significantly smaller than that released in solution of pH=7.0. All the results suggest that nanosized hydroxyapatite particles could be successfully used as controlled released carrier of protein.
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| 关 键 词： | 纳米材料 羟基磷灰石颗粒 蛋白质吸附 可控性药物释放 |
| 收稿时间： | 21 June 2006. |
| 修稿时间： | 2006-06-21 |
Protein Adsorption onto Nanosized Hydroxyapatite Particles for Controlled Drug Release |
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| WANG Bo TENG Li-rong WANG Chun-yan MENG Qing-fan ZHAO Ling-zhi GAO Bo.Protein Adsorption onto Nanosized Hydroxyapatite Particles for Controlled Drug Release[J].Chemical Research in Chinese University,2007,23(3):254-257. |
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| Authors: | Bo WANG Li-rong TENG Chun-yan WANG Qing-fan MENG Ling-zhi ZHAO Bo GAO |
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| Institution: | aCollege of Life Science;bState Key Laboratory of Inorganic Synthesis and Preparative Chemistry, Jilin University, Changchun 130012, P. R. China |
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| Abstract: | Nanosized hydroxyapatite(nsHAp) was synthesized to examine its possibility as a controlled release carrier of protein. To achieve effective protein release from nanosized hydroxyapatite, the study of the adsorptive properties of protein on nsHAp and different influence parameters such as pH, calcium, and phosphate concentrations during the adsorption process is necessary. Ovalbumin(OVA) was selected as the model of growth factors. The results show that the amount of OVA adsorbed onto nsHAp in acetic buffer(pH=3.6) is more than that in acetic buffer(pH=5.6) because of the electric interaction. The amount of OVA adsorption in phosphate buffer solution(PBS) is smaller than that in acetic buffer because of surface complexation and surface hydroxylation. The presence of Ca2 dramatically increases the adsorbed amount of OVA in acetic buffer on maintaining the same pH. Meanwhile, the release kinetics of OVA adsorbed onto nsHAp(nsHAp-OVA) was also examined. The amount of released OVA in PBS(pH=5.6) was significantly smaller than that released in solution of pH=7.0. All the results suggest that nanosized hydroxyapatite particles could be successfully used as controlled released carrier of protein. |
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| Keywords: | Nanomaterials Hydroxyapatite Ovalbumin Controlled release |
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