高效液相色谱β-环糊精键合固定相直接拆分硫代和硒代缩水甘油醚对映异构体

 用两种不同的方法合成了β-环糊精键合固定相。通过对硝基苯胺异物体的拆分，对键合相进行了评价。拆分了3种硫代缩水甘油醚和2种未见文献报道的硒代缩水甘油醚。

Chiral separation of thioglycidyl ether and glycidylselenide on cyclodextrin bonded stationary phase via high performance liquid chromatography]

beta- Cyclodextrin bonded chiral stationary phases (CD-CSP) were prepared after the method of Armstrong (CD-CSP I) and of Huang Tianbao (CD-CSP II). With the separation of o-, m- p-nitroaniline, it was found that the chromatographic properties of CD-CSP II was superior to that of CD-CSP I. Three racemes of thioglycidyl ethers and two racemes of glycidylselenides were resolved on CD-CSP II via high performance liquid chromatography. The mobile phase consisted of different volume ratios of methanol/0.3% triethylammonium acetate buffer solution(pH 5), and the eluate was detected with a UV-visible detector at a wavelength of 254 nm. With the increase of methanol volume ratio in the mobile phase, the retention time decreased rapidly, but within the range of methanol valume ratio 40/100 to 50/100, the separation factor only decreased slightly. The racemes examined contained aromatic groups and hydroxy groups which can form inclusion complexes and hydrogen bonding interactions with beta-cyclodextrin. The chiral separation of the racemes is successful with the mobile phase of V(methanol):V(buffer) = 40:60.