| 液相色谱-串联质谱法测定尿液中的泛酸 |
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| 引用本文： | 张小涛,章云飞,侯宏卫,陈欢,刘勇,王安,胡清源.液相色谱-串联质谱法测定尿液中的泛酸[J].分析测试学报,2017,36(6):809-811. |
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| 作者姓名： | 张小涛 章云飞 侯宏卫 陈欢 刘勇 王安 胡清源 |
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| 作者单位： | 1. 国家烟草质量监督检验中心,河南郑州450001;中国科学院合肥物质科学院应用技术所,安徽合肥230031;2. 河南省肿瘤医院,河南郑州,450008;3. 国家烟草质量监督检验中心,河南郑州,450001;4. 中国科学院合肥物质科学院应用技术所,安徽合肥,230031 |
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| 基金项目： | 国家自然科学基金项目(21277174) |
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| 摘 要： | 该文建立了检测尿液中泛酸含量的液相色谱-串联质谱(HPLC-MS/MS)方法,尿液经过离心、稀释后,采用ACPUITY UPLC SS T3(2.1 mm×100 mm,1.8μm)色谱柱进行分离,电喷雾正离子模式电离,多反应监测模式进行检测,方法的线性关系良好(r=0.999 3),方法检出限为0.46 ng/m L,回收率为87.9%~95.3%,相对标准偏差(RSD)为2.5%~13.0%。该方法具有灵敏度高、分析时间短等特点,可用于尿液中泛酸含量的分析。
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| 关 键 词： | 液相色谱-串联质谱 尿液 泛酸 生物监测 |
Determination of Pantothenic Acid in Human Urine by Liquid Chromatography- Tandem Mass Spectrometry |
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| ZHANG Xiao-tao,ZHANG Yun-fei,HOU Hong-wei,CHEN Huan,LIU Yong,WANG An,HU Qing-yuan.Determination of Pantothenic Acid in Human Urine by Liquid Chromatography- Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2017,36(6):809-811. |
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| Authors: | ZHANG Xiao-tao ZHANG Yun-fei HOU Hong-wei CHEN Huan LIU Yong WANG An HU Qing-yuan |
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| Abstract: | A liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method for the determination of pantothenic acid in urine was developed and validated.After centrifugation and dilution,the sample was separated on an ACPUITY UPLC HSS T3(2.1 mm× 100 mm,1.8 μm) column produced by the Waters corporation,and ionized in electro spray positive ion mode,then detected using multi-reaction monitoring mode.The calibration curve has a good linear range with a correlation coefficient(r) of 0.999 3.The limit of detection is 0.46 ng/mL,the recoveries are in the range of 87.9％-95.3％,and the RSDs are in the range of 2.5％-13.0％.The method has the advantages of high sensitivity and short analysis time,and could be used to analyze pantothenic acid in human urine. |
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| Keywords: | liquid chromatography tandem mass spectrometry urine pantothenic acid biomonitoring |
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