Fabrication and properties of mineralized collagen‐chitosan/hydroxyapatite scaffolds |
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Authors: | Haiguang Zhao Lie Ma Changyou Gao Jiacong Shen |
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Institution: | Department of Polymer Science and Engineering, Zhejiang University, Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of Education, Hangzhou 310027, China |
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Abstract: | A hydroxyapatite (HAp)/biopolymer composite scaffold was fabricated by mineralizing a crosslinked collagen/chitosan, which was pre‐mineralized with Ca2+ and phosphate salts, in simulated body fluid (SBF) for only 24 hr. A self‐organized structure similar to bone is expected. Microstructures of the crosslinked collagen/chitosan scaffold, the pre‐mineralized collagen–chitosan scaffold (CCS), and the mineralized collagen‐chitosan/HAp scaffolds (MCCHS) were characterized by scanning electron microscopy (SEM), revealing non‐alteration of the porous structure and formation of the HAp particles. X‐ray diffractometer (XRD) confirmed the crystalline structure of the HAp. Thermal gravimetric analysis found that more HAp particles were formed when the CCSs were pre‐mineralized in a higher concentration of Ca2+. Water‐uptake ratio of the crosslinked CCS was ~160, decreased to ~120 after incubating in Ca2+ solution, and further decreased to ~20 after mineralization. Mechanical strength of the CCS was improved significantly after the in situ mineralization too. The method introduced here may be potentially applied to obtain other biopolymer/HAp composite in a short period. Copyright © 2008 John Wiley & Sons, Ltd. |
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Keywords: | collagen chitosan biomaterials hydroxyapatite biomineralization |
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