| A Novel Fluorescent Reagent for Analysis of Hydrogen Peroxide |
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| 作者姓名： | 董素英 苏美红 聂丽华 马会民 |
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| 作者单位： | Laboratory of Chemical Biology，Center for Molecular Sciences，Institute of Chemistry，Chinese Academy of Sciences，Beijing 100080，China，Laboratory of Chemical Biology，Center for Molecular Sciences，Institute of Chemistry，Chinese Academy of Sciences，Beijing 100080，China，Laboratory of Chemical Biology，Center for Molecular Sciences，Institute of Chemistry，Chinese Academy of Sciences，Beijing 100080，China，Laboratory of Chemical Biology，Center for Molecular Sciences，Institute of Chemistry，Chinese Academy of Sciences，Beijing 100080，China |
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| 基金项目： | ProjectsupportedbytheNationalNaturalScienceFoundationofChina (Nos .2 0 175 0 3 1and 2 0 0 3 5 0 10 )andCMS CX2 0 0 10 4ofCAS . |
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| 摘 要： | IntroductionTheoxidationofmanyclinicalsubstancesinbodyfluidsproducesaquantityofhydrogenperoxide ,sothedetermina tionoftracehydrogenperoxideisofconsiderableimportanceinclinicalchemistry .1Further,themonitoringofhydrogenperoxideisalsonecessarytoenvironmentalsciencesinceitisakeyspeciesinthereactionsofthetroposphere,beingin volvedinimportantreactionssuchasthecatalyzedoruncat alyzedaqueousphaseoxidationofSO2 andtheultraviolet en hancedaqueousphaseoxidationoforganicspecies.2 Uptonow ,variousmethods…
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| 关 键 词： | 体液 血清 过氧化氢 分析方法 荧光试剂 |
A Novel Fluorescent Reagent for Analysis of Hydrogen Peroxide |
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| Dong Su‐Ying,Su Mei‐Hong,Nie Li‐Hua,Ma Hui‐Min.A Novel Fluorescent Reagent for Analysis of Hydrogen Peroxide[J].Chinese Journal of Chemistry,2003,21(2):189-191. |
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| Authors: | Dong Su‐Ying Su Mei‐Hong Nie Li‐Hua Ma Hui‐Min |
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| Abstract: | 8‐(4,6‐Dichloro‐1,3,5‐triazinoxy)quinoline (DTQ) was evaluated as a new fluorescent reagent for determining hydrogen peroxide. It was found that the fluorescence intensity of DTQ in alkaline medium could be dramatically enhanced upon addition of H2Q2. Based on this effect, a simple and selective method for the spectrofluorimetric determination of hydrogen peroxide was established. The relative standard deviation of the method was found to be 1.1% for 9 replicate determinations of a 4.6 × 10–6 mol/L hydrogen peroxide solution. The linear range was 2.3 × 10–7—2.3 × 10–5 mol/L with a detection limit of 2.2 × 10–8 mol/L (S/N = 3). The method was attempted to determine hydrogen peroxide in synthetic human serum samples with satisfactory results. |
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| Keywords: | dichloro 1 3 5 triazinoxy)quinoline hydrogen peroxide serum fluorescence |
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