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| 规模化蛋白质生物质谱鉴定中肽段氨基端环化修饰现象 | |
| 引用本文： | 卢庄,赵丽艳,张养军,蔡耘,邓玉林,张玉奎,钱小红.规模化蛋白质生物质谱鉴定中肽段氨基端环化修饰现象[J].分析化学,2009,37(7). |
| 作者姓名： | 卢庄 赵丽艳 张养军 蔡耘 邓玉林 张玉奎 钱小红 |
| 作者单位： | 1. 北京理工大学生命科学与技术学院,北京,100081;蛋白质组学国家重点实验室,北京蛋白质组研究中心,北京放射医学研究所,北京,102206 2. 蛋白质组学国家重点实验室,北京蛋白质组研究中心,北京放射医学研究所,北京,102206 3. 北京理工大学生命科学与技术学院,北京,100081 |
| 基金项目： | 国家自然科学基金，国家重点基础研究发展规划(973计划) |
| 摘 要： | 对蛋白质样品制备中引入的氨基酸残基的一种现象--蛋白质酶切肽段氨基端的环化修饰现象的初步研究结果显示,很多以谷氨酰胺(Q)或氨乙酰化修饰的半胱氨酸(CAM_C)残基起始的肽段会发生氨基端的环化修饰,且修饰反应不完全,在同一样本中修饰与非修饰两种状态常同时存在,并且环化修饰后的肽段的反相色谱保留时间发生延迟.在数据库检索时添加环化修饰,可以提高蛋白质的鉴定成功率.本研究结果为大规模的蛋白质质谱数据解析提供了有价值的参考. |
| 关 键 词： | 肽段氨基端 环化 规模化 蛋白质鉴定 |
N-Terminal Cyclization of Peptides in Large-scale Protein Identification Based on Biological Mass Spectrometry |
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| LU Zhuang,ZHAO Li-Yan,ZHANG Yang-Jun,CAI Yun,DENG Yu-Lin,ZHANG Yu-Kui,QIAN Xiao-Hong.N-Terminal Cyclization of Peptides in Large-scale Protein Identification Based on Biological Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2009,37(7). | |
| Authors: | LU Zhuang ZHAO Li-Yan ZHANG Yang-Jun CAI Yun DENG Yu-Lin ZHANG Yu-Kui QIAN Xiao-Hong |
| Institution: | LU Zhuang1,2,ZHAO Li-Yan2,ZHANG Yang-Jun2,CAI Yun2,DENG Yu-Lin1,ZHANG Yu-Kui1,QIAN Xiao-Hong1,2 1(School of Life Science & Technology,Beijing Institute of Technology,Beijing 100081)2(State Key Laboratory of Proteomics-Beijing Proteome Research Center-Beijing Institute of Radiation Medicine,Beijing 102206) |
| Abstract: | Biological mass spectrometry has been developed for the large-scale protein identification.The successful identification of protein in proteomic study is based on an effective match of MS data to the sequence in database.Because of the diversity and heterogeneity of protein modification,the experimental data obtained by mass spectrometry does not match the theoretical value sometimes,which makes about 90 percent or more of the tandem mass spectra not be effectively identified.This has become one of the most... |
| Keywords: | N-Terminal of peptides cyclization largE-scale protein identification |
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