Preparation for nitrocellulose membrane-poly (vinyl alcohol)-ionic imprinting and its application to determine trace copper by room temperature phosphorimetry |
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Authors: | Li Zhi-Ming Liu Jia-Ming Liu Zhen-Bo Liu Qin-Ying Lin Xuan Li Fei-Ming Yang Min-Lan Zhu Guo-Hui Huang Xiao-Mei |
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Institution: | a Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou 363000, PR China b Department of Orthopedics and Traumatology, Fujian College of Traditional Chinese Medicine, Fuzhou 350003, PR China c Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou 363000, PR China |
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Abstract: | Nitrocellulose membrane-poly (vinyl alcohol)-ionic imprinting (NCM-PVA-I-I) was prepared using Cu2+ as template. The cavity in NCM-PVA-I-I matched Cu2+ very well and the selectivity was high. Cu2+ entered the cavity and then could form ionic association (Cu2+]·(Fin−)2]) with the anion of fluorescein (Fin−) outside the cavity by electrostatic effect. Cu2+]·(Fin−)2] could emit strong and stable room temperature phosphorescence on NCM-PVA-I-I. Its ΔIp was proportional to the content of Cu2+. Based on the above facts, a new method for the determination of trace copper by solid substrate-room temperature phosphorimetry (NCM-PVA-I-I-SS-RTP, SS-RTP is the abbreviation of solid substrate-room temperature phosphorimetry) using NCM-PVA-I-I technique has been established. The linear range of this method was 2.00-144.00 fg Cu2+ spot−1 (sample volume: 0.40 μL spot−1, corresponding concentration: 5.00-360.00 pg mL−1), and the detection limit calculated by 3Sb/k was 0.43 fg Cu2+ spot−1 (corresponding concentration: 1.1 × 10−12 g mL−1, n = 11). Samples containing 2.00 and 144.00 fg Cu2+spot−1 were measured, respectively, for seven times and R.S.D.s were 3.5% and 4.7%. NCM-PVA-I-I-SS-RTP could combine very well the characteristics of both the high sensitivity of SS-RTP and the high match and selectivity of NCM-PVA-I-I, and it was rapid, accurate, sensitive and with good repeatability. It has been successfully applied to determine trace copper in human hair and tea samples. |
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Keywords: | Copper Nitrocellulose membrane-poly (vinyl alcohol)-ionic imprinting technique Solid substrate-room temperature phosphorimetry |
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