Simultaneous determination of pethidine and methadone by capillary electrophoresis with electrochemiluminescence detection of tris(2,2′-bipyridyl)ruthenium(II) |
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Authors: | Bingyan Han Yan Du Erkang Wang |
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Institution: | aState Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China;bGraduate School of the Chinese Academy of Sciences, Beijing 100039, PR China |
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Abstract: | In this paper, we described a simple and rapid method, capillary electrophoresis with electrochemiluminescence (CE–ECL) detection using tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+), to simultaneously detect pethidine and methadone. Analytes were injected to separation capillary of 67.5 cm length (25 μm i.d., 360 μm o.d.) by electrokinetic injection for 10 s at 10 kV. Under the optimized conditions: ECL detection at 1.20 V, 30 mM sodium phosphate (pH 6.0) as running buffer, separation voltage at 14.0 kV, 5 mM Ru(bpy)32+ with 50 mM sodium phosphate (pH 6.5) in the detection cell, the linear range from 2.0 × 10− 6 to 2.0 × 10− 5 M for pethidine and 5.0 × 10− 6 to 2.0 × 10− 4 M for methadone and detection limits of 0.5 μM for both of them were achieved (S/N = 3). Relative standard derivations of the ECL intensity were 2.09% and 6.59% for pethidine and methadone, respectively. |
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Keywords: | Capillary electrophoresis Electrochemiluminescence Pethidine Methadone |
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