一种红光发射的粘度荧光探针

以三苯胺和苯并噻唑盐为原料,设计合成了一种具有红光发射特性的D-π-A型荧光粘度探针N-乙酸乙酯基-2-(4-甲酸甲酯基三苯胺-4'-乙烯基)苯并噻唑六氟磷酸盐(L),运用现代分析测试手段进行了系统地表征。 研究结果表明,探针L的最大发射波长为630 nm,能有效地降低生物背景,提高生物成像的信噪比。 该探针对粘度有很好的荧光响应,其荧光强度比值(I/I₀)的对数与粘度的对数呈现很好的线性关系(R²=0.9934)。 此外,探针L对极性的敏感性小,且荧光信号不受生物分子的干扰。 生物学研究结果表明,探针L具有低的细胞毒性,可应用于细胞内微环境粘度的荧光成像。

Synthesis and Properties of Red-EmittingFluorescence Probe for Viscosity Detection

A red-emitting D-π-A type fluorescence probe(E)-3-(2-ethoxy-2-oxoethyl)-2-(4-((4-(methoxycarbonyl)phenyl)(phenyl)amino)styryl)benzod]thiazol-3-ium hexafluorophosphate salt(L)based on triphenylamine and benzothiazole salts for detecting viscosity was designed and synthesized,which was fully characterized by modern analytical testing methods.The systematic investigations indicated that probe L displayed a strong fluorescence emission at about 630 nm in viscous media,which could minimize the background noises effectively and improve the signal-to-noise(S/N)ratio of biological imaging.Probe L exhibited great sensitivity to viscosity.Moreover,probe L showed a good linear relationship(R 2=0.9934)between the fluorescence intensity response(log(I/I 0))and viscosity(logη).Besides,probe L exhibited a good selectivity and could serve as a fluorescent probe for sensing viscosity without being interfered by polarity and other analytes.Furthermore,biological experimental indicated that probe L possesses low cytotoxicity,which can be applied to the fluorescence imaging of intracellular microenvironment viscosity.