羟基磷灰石与牛血清白蛋白相互作用的原位红外光谱研究

应用原位(in situ)全反射红外光谱研究牛血清白蛋白在电化学法制备的羟基磷灰石表面的吸附和成键行为, 探索电化学法制备的HA生物材料/生物环境界面过程和生物相容性的微观本质.

In Situ ATR-FTIR Study on the Interaction of HA with Bovine Serum Albumin

In situ ATR-FTIR was used to study the interaction of bovine serum albumin(BSA) and hydroxyapatite(HA) in this work.Both on the electrochemically deposited HA and self assemblied titanate nanotubes(TNT) substrates,the adsorption of BSA increases with the increase of time,and its adsorption on HA surface is much larger than that on TNT surface,implying that HA has a good biocompatibility as a biomaterial.It is indicated that Ca~(2+) plays an essential role in the protein absorption on the HA surface,due to that BSA is negatively charged,and it is able to combine with cation locations in HA particles by electrostatic forces.It is found that,when reacting with BSA,the phosphate band of HA in solution shifts to higher wavenumbers,indicating that PO~(3-)_(4) can also be an adsorption site for BSA.The spectroscopic study on the interaction between HA and BSA will be helpful for further understanding the nano hydroxyapatite bioproperties on molecular level.