Electrochemiluminescence Biosensor for Glucose Based on Graphene/Nafion/GOD Film Modified Glassy Carbon Electrode |
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| Authors: | Xiaoping Chen Huazhen Ye Wanzong Wang Bin Qiu Zhenyu Lin Guonan Chen |
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| Institution: | 1. Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, P.?R. China fax: 86‐591‐22866135;2. Department of Orthopedics, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, P.?R. China |
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| Abstract: | Glucose oxidase(GOD) was encapsulated in the Graphene/Nafion film modified glassy carbon electrode(GCE) and used as an ECL sensor for glucose. The GOD retains its bioactivity after being immobilized into the composite film. The sensor gives a linear response for glucose in the range of 2.0×10?6–1.0×10?4 mol/L with a detection limit of 1.0×10?6 mol/L. The sensor showed good stability, the RSD for continuous scanning for 5.0×10?5 mol/L glucose was 4.21 % (n=5). After being stored in 0.05 mol/L pH 7.4 PBS in 4 °C for two weeks, the modified electrode maintains 80 % of its initial activity. The glucose sensor provides new opportunity for clinical diagnosis applications. |
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| Keywords: | Graphene Glucose Electrochemiluminescence Biosensors |
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