Forensic analysis of dyed textile fibers |
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Authors: | John V Goodpaster Elisa A Liszewski |
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Institution: | (1) MOE Key Laboratory of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei, 430030, China;(2) Hubei Entry–Exit Inspection and Quarantine Bureau of PRC, Wuhan, Hubei, 430022, China |
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Abstract: | A rapid, specific, and sensitive method has been developed using molecularly imprinted polymers (MIPs) as solid-phase extraction
sorbents for extraction of trace tetracycline antibiotics (TCs) in foodstuffs. MIPs were prepared by precipitation polymerization
using tetracycline as the template. Under the optimal condition, the imprinting factors for MIPs were 4.1 (oxytetracycline),
7.0 (tetracycline), 7.4 (chlortetracycline), 7.7 (doxycycline), respectively. Furthermore, the performance of MIPs as solid-phase
extraction sorbents was evaluated and high extraction efficiency of molecularly imprinted solid-phase extraction (MISPE) procedure
was demonstrated. Compared with commercial sorbents, MISPE gave a better cleanup efficiency than C18 cartridge and a higher
recovery than Oasis HLB cartridge. Finally, the method of liquid chromatography–tandem mass spectrometry coupled with molecular-imprinted
solid-phase extraction was validated in real samples including lobster, duck, honey, and egg. The spiked recoveries of TCs
ranged from 94.51% to 103.0%. The limits of detection were in the range of 0.1–0.3 μg kg−1.
Chromatograms obtained by direct injection of the spiked egg extracts (5 × 10-3 mmol L−1) and purification with MISPE |
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Keywords: | Tetracycline antibiotics Precipitation polymerization Molecularly imprinted solid-phase extraction Foodstuff Liquid chromatography– tandem mass spectrometry |
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